Selenium mediated dose-inhibition of 7,12-dimethylbenz[a] anthracene-induced transformation of mammary cells in organ culture.
Selenium mediated dose-inhibition of 7,12-dimethylbenz[a] anthracene-induced transformation of mammary cells in organ culture.
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硒介导的7,12-二甲基苯并[a]蒽诱导的器官培养中乳腺细胞转化的剂量抑制。
DOI：
10.1016/0304-3835(82)90031-3
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发表时间：
1982
期刊：
影响因子：
9.7
通讯作者：
Banerjee,MR
中科院分区：
文献类型：
--
作者：
Chatterjee,M;Banerjee,MR
Influence of selenium (sodium selenite, Na 2 SeO 3) on transformation of mammary cells, induced by 7, 12-dimethylbenz [a] anthracene (DMBA) was assessed in organ culture of the whole mammary glands from BALB c female mice. Transformation was determined by the presence of nodule-like alveolar lesions (NLAL) induced by DMBA in the glands in vitro. Selenium at concentrations of 10− 8 M and 10− 7 M enhanced the transformation frequency of the glands, acting both at the ‘initiation’and ‘promotional’stages. The enhancement was notable both by scoring the frequency of glands with NLAL and the number of NLAL per gland. At selenium concentration of 10− 5 M a modest 18% inhibition of the frequency of transformed glands was detectable at the initiation stage. At promotional stage 10− 6 M selenium caused a 37% inhibition of the frequency of transformed glands and at the same stage, an 84% inhibition was present at 10− 5 M concentration of selenium in the medium. The concentration of 10− 4 M was toxic to the glands in vitro. The inhibition of the frequency of the transformed glands was accompanied by a pronounced reduction of the number of NLAL per gland. Thus, selenium is capable of causing a dose-dependent inhibition of transformation of mammary epithelial cells in organ culture, acting mostly at the promotional stage of the process. Selenium appears to act by preventing expression of the transformed cells as ‘high risk’, potentially neoplastic lesions in the glands in vitro. The present findings thus provide an in vitro model for future studies on the mechanism of selenium mediated chemoprevention of the neoplastic process.
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DOI：
--
发表时间：
1981
期刊：
Journal of the National Cancer Institute
影响因子：
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作者：
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影响因子：
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影响因子：
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通讯作者：
A. Griffin
DOI：
--
发表时间：
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期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
R. Shamberger
通讯作者：
R. Shamberger