Genome-wide impacts of alien chromatin introgression on wheat gene transcriptions
Genome-wide impacts of alien chromatin introgression on wheat gene transcriptions
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外来染色质渗入对小麦基因转录的全基因组影响
DOI：
10.1038/s41598-020-61888-1
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发表时间：
2020-03-16
影响因子：
4.6
通讯作者：
Liu, Wenxuan
中科院分区：
文献类型：
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作者：
Dong, Zhenjie;Ma, Chao;Liu, Wenxuan
小麦-异源渐渗可显著改善六倍体小麦的农艺性状和对生物和非生物胁迫的耐受性。然而，外源基因在小麦遗传背景中的转录水平的相互作用却很少被研究。在这项研究中，我们报告了来自Ae的渐渗染色体的全基因组影响。小麦地方品种中国春的longissimaon基因转移。RNA-seq分析表明，与CS相比，CS-Ae.longissimadisomic 3Sl#2（3B）替代系TA 3575和二体6Sl#3附加系TA 7548中分别有5.37%和4.30%的基因显着差异表达（DEG）。此外，561个DEG，包括413个上调和148个下调或不转录的基因，同时受到渗入染色体3S1#2和6S1#3的影响，占TA 3575和TA 7548中DEG的41.25%和38.79%。17个DEG，注释为R基因，由携带染色体3S1#2和6S1#3的渐渗系共享，其赋予对白粉病的抗性。本研究将有助于了解小麦对外源基因或染色体导入的反应以及由3S1#2和6S1#3染色体上的抗白粉病基因启动的植物防御反应。
Agronomic characteristics and tolerance to biotic and abiotic stresses in hexaploid wheat can be drastically improved through wheat-alien introgression. However, the transcriptional level interactions of introduced alien genes in the wheat genetic background is rarely investigated. In this study, we report the genome-wide impacts of introgressed chromosomes derived fromAe. longissimaon gene transcriptions of the wheat landrace Chinese Spring. RNA-seq analyses demonstrated 5.37% and 4.30% of the genes were significantly differentially expressed (DEGs) in CS-Ae.longissimadisomic 3Sl#2(3B) substitution line TA3575 and disomic 6Sl#3 addition line TA7548, respectively when compared to CS. In addition, 561 DEGs, including 413 up-regulated and 148 down-regulated or not transcribed genes, were simultaneously impacted by introgressed chromosomes 3Sl#2 and 6Sl#3, which accounts for 41.25% of the DEGs in TA3575 and 38.79% in TA7548. Seventeen DEGs, annotated as R genes, were shared by both introgression lines carrying chromosomes 3Sl#2 and 6Sl#3, which confer resistance to powdery mildew. This study will benefit the understanding of the wheat gene responses as result of alien gene(s) or chromosome intogression and the plant defense response initiated by powdery mildew resistance genes in chromosomes 3Sl#2 and 6Sl#3.