Epitopes recognized by the neutralizing antibodies of an HIV-1-infected individual.
Epitopes recognized by the neutralizing antibodies of an HIV-1-infected individual.
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HIV-1 感染者的中和抗体识别的表位。
DOI：
10.4049/jimmunol.144.12.4641
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发表时间：
1990
影响因子：
4.4
通讯作者：
S. Putney
中科院分区：
文献类型：
--
作者：
A. Profy;P. Salinas;L. Eckler;N. Dunlop;P. Nara;S. Putney
来自HIV-1感染者的血清通常中和多种病毒分离株。为了确定这些中和抗体识别包膜蛋白gp 120的V3区（氨基酸308-332）中的主要中和决定簇的程度，通过亲和色谱法在固定化肽柱上分离一种广泛中和的血清。中和一种分离株（HTLV-IIIMN）的抗体基本上但不完全被对应于其V3决定簇的一部分的肽吸收，而中和另外两种分离株（HTLV-IIIB和HTLV-IIIRF）的抗体不被对应于其中和决定簇的同源肽吸收。中和抗体也不能被全长包膜蛋白gp 160和先前报道为广泛中和表位（氨基酸254-274和735-752）的另外两种包膜肽吸收。我们的结论是，受感染的个人提出了一个类型限制的中和反应，针对线性表位在V3区，广泛的中和识别表位尚未确定。
Sera from individuals infected by HIV-1 usually neutralize multiple viral isolates. To determine the extent to which these neutralizing antibodies recognize a principal neutralizing determinant in the V3 region of the envelope protein gp120 (amino acids 308-332), one broadly neutralizing serum was fractionated by affinity chromatography on immobilized peptide columns. Antibodies that neutralize one isolate (HTLV-IIIMN) were substantially but not completely absorbed by the peptide corresponding to a portion of its V3 determinant, whereas the antibodies that neutralize two other isolates (HTLV-IIIB and HTLV-IIIRF) were not absorbed by homologous peptides corresponding to their neutralizing determinants. Neutralizing antibodies also failed to be absorbed by full length envelope protein gp160 and by two other envelope peptides previously reported to be broadly neutralizing epitopes (amino acids 254-274 and 735-752). We conclude that the infected individual had raised a type-restricted neutralizing response targeted at a linear epitope in the V3 region, and that broad neutralization resulted from recognition of epitopes not yet identified.