Industrial‐scale fabrication of an osteogenic and antibacterial PLA/silver‐loaded calcium phosphate composite with significantly reduced cytotoxicity
Industrial‐scale fabrication of an osteogenic and antibacterial PLA/silver‐loaded calcium phosphate composite with significantly reduced cytotoxicity
复制标题
工业规模制造成骨抗菌 PLA/载银磷酸钙复合材料，且细胞毒性显着降低
DOI：
10.1002/jbm.b.34185
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Loboa, Elizabeth G.
中科院分区：
文献类型：
--
作者：
Cai, Shaobo;Pourdeyhimi, Behnam;Loboa, Elizabeth G.
在这项研究中，我们报告了一种多功能聚合物复合材料作为骨组织工程支架材料的工业规模制造方法。这项研究成功地展示了应用工业聚合物加工技术生产特殊功能化组织工程支架的潜力。通过引入一种新合成的多功能添加剂--银掺杂磷酸钙（银-CaP），该复合材料具有良好的人脂肪干细胞（hASC）成骨诱导作用，并对大肠杆菌和金黄色葡萄球菌具有良好的抗菌效果。此外，相对于先前报道的将银颗粒直接装载到聚合物材料中的方法，我们的复合材料表现出显著降低的银相关的细胞毒性。增强的生物相容性可能是用于再生医学应用的材料的显著优势，其中临床安全性是主要考虑因素。还研究了不同银负载方法对hASC成骨分化的影响。总的来说，这项研究的结果表明了一种有前途的替代方法，可以在工业规模上生产多功能支架，具有更高的通量，更低的成本和更高的重现性。© 2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107 B：900-910，2019。
In this study, we report an industrial‐scale fabrication method of a multifunctional polymer composite as a scaffold material for bone tissue engineering. This study successfully demonstrated the potential of applying industrial polymer processing technologies to produce specially functionalized tissue engineering scaffolds. With the inclusion of a newly synthesized multifunctional additive, silver‐doped‐calcium phosphate (silver‐CaP), the composite material exhibited excellent osteogenic inducibility of human adipose‐derived stem cells (hASC) and satisfactory antibacterial efficacy againstEscherichia coliandStaphylococcus aureus. Also, relative to previously reported methods of direct loading silver particles into polymeric materials, our composite exhibited significantly reduced silver associated cytotoxicity. The enhanced biocompatibility could be a significant advantage for materials to be used for regenerative medicine applications where clinical safety is a major consideration. The impact of different silver loading methodologies on hASC’ osteogenic differentiation was also studied. Overall, the results of this study indicate a promising alternative approach to produce multifunctional scaffolds at industrial‐scale with higher throughput, lower cost, and enhanced reproducibility. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 900–910, 2019.