Liming does not counteract the influence of long-term fertilization on soil bacterial community structure and its co-occurrence pattern
Liming does not counteract the influence of long-term fertilization on soil bacterial community structure and its co-occurrence pattern
复制标题
DOI：
10.1016/j.soilbio.2018.05.003
复制
发表时间：
2018-08-01
影响因子：
9.7
通讯作者：
Dyck, Miles F.
中科院分区：
文献类型：
--
作者：
Ma, Bin;Lv, Xiaofei;Dyck, Miles F.
化肥是提高作物产量的一种常用农艺措施，石灰常被用来抵消长期施肥引起的土壤酸化，但石灰是否能抵消长期施肥对土壤细菌群落结构及其共生格局的影响尚不清楚。通过16SRRNA基因扩增序列测定，研究了长期施肥和施石灰(L)对土壤细菌群落结构及其共生网络的影响。结果表明，长期施石灰对土壤pH的影响有一定的抵消作用，但对土壤可溶性有机碳(SOC)和电导率(EC)的影响不明显。长期施肥影响了0~10 cm土壤中的酸杆菌、变形杆菌和伽马变形杆菌的丰度，影响了10~20 cm土壤中的变形杆菌、变形杆菌和双歧杆菌的丰度，石灰影响了0~10 cm土壤中的酸杆菌、变形杆菌、氯杆菌和10~20 cm土壤中的变形杆菌的丰度。氮磷钾肥-L处理的土壤细菌群落结构与其他处理不同，主要受土壤有机碳、电导率和NO3-浓度的影响。长期施肥或单独施石灰降低了细菌共生网络中的连接数，而NPKS-L处理使细菌共生网络中的连接数增加。尽管施石灰减轻了施肥引起的土壤酸化，但本研究表明，施石灰并没有抵消长期施肥对土壤细菌群落结构及其共生格局的影响，因此不能恢复长期施肥改变的土壤微生物功能。
Chemical fertilizer application is a common agronomic practice to improve crop productivity and liming is often used to counteract the soil acidification caused by long-term fertilization; however, it is unclear whether liming will counteract the effect of long-term fertilization on soil bacterial community structure and its co-occurrence pattern. Here, we examined the influence of long-term fertilization (NPKS) and liming (L) on soil bacterial community structure and its co-occurrence networks by sequencing 16S rRNA gene amplicons. Our results showed that liming counteracted the influence of long-term fertilization on soil pH, but not on soluble organic carbon (SOC) and electrical conductivity (EC). Long-term fertilization affected the abundance of Acidobacteriia, Deltaproteobacteria and Gammaproteobacteria in the 0-10 cm soil, and that of Deltaproteobacteria, Gammaproteobacteria and Gemmatimonadetes in the 10-20 cm soil; whereas liming affected the abundance of Acidobacteriia, Gammaproteobacteria, and Chloracidobacteria in the 0-10 cm soil and that of Deltaproteobacteria in the 10-20 cm soil. The bacterial community structure in soils with the NPKS-L treatment was different from that with other treatments, and was mainly affected by SOC, EC, and NO3- concentration. The link numbers in bacterial co-occurrence networks were decreased by long-term fertilization or liming alone, but were increased by the NPKS-L treatment. Notwithstanding the fact that liming alleviates fertilization-induced soil acidification, this study indicates that liming did not counteract the effect of long-term fertilization on soil bacterial community structure and its co-occurrence pattern, hence cannot recuperate the soil microbial functionality that is changed by long-term fertilization.