Bacteriophage T4 transfer RNA. II. Mutants of T4 defective in the formation of functional suppressor transfer RNA.
Bacteriophage T4 transfer RNA. II. Mutants of T4 defective in the formation of functional suppressor transfer RNA.
复制标题
噬菌体 T4 转移 RNA。
DOI：
10.1016/0022-2836(72)90023-x
复制
发表时间：
1972
影响因子：
5.6
通讯作者：
J. Abelson
中科院分区：
文献类型：
--
作者：
J. H. Wilson;J. Abelson
151 independent suppressor-negative derivatives of a T4-coded nonsense suppressor,psub+, have been isolated and characterized. They fall into two complementation groups. One, designatedmb(modifier ofpsub+phenotype), is unlinked topsub+and has been located about 10 map units fromrII. The second group, designatedpsub−, includes deletions and single base changes which affect sites within 0.2 map units of the original mutation. The secondary mutations in thosepsub−mutants which still contain the original mutation,psub, have been positioned within the gene for the serine-transfer RNA by a series of two- and threefactor genetic crosses.32P-labeled tRNA frommb,psub−and wild-type infected cells has been compared by polyacrylamide gel electrophoresis. Inmb-infected cells several of the tRNA species are missing, while inpsub−-infected cells only the ser-tRNA is clearly absent.These studies suggest there are only two phage genes which are essential for the production of functional ser-tRNA. One is the structural gene for the ser-tRNA and the second plays an undefined role which affects several tRNA's.
