Rhinovirus-induced interferon-γ and airway responsiveness in asthma
Rhinovirus-induced interferon-γ and airway responsiveness in asthma
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DOI：
10.1164/rccm.200306-737oc
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发表时间：
2003-11-01
影响因子：
24.7
通讯作者：
Busse, WW
中科院分区：
文献类型：
--
作者：
Brooks, GD;Buchta, KA;Busse, WW
大多数哮喘急性发作是由呼吸道感染引起的，鼻病毒（RV）是最常见的病毒。最近的证据表明，IFN-γ的产生减少与更严重的感冒和病毒清除延迟有关。IFN-γ的产生是否也与哮喘严重程度的一般特征有任何关系尚未确定。为了评估这一假设，将19名特应性和哮喘受试者的外周血单核细胞与RV 16孵育6天，以确定IFN-γ和白细胞介素（IL）-5的产生;然后将这些反应与气流阻塞和气道反应性的测量结果进行比较。RV 16诱导的IFN-γ产生与乙酰甲胆碱PD显著相关（r = 0.50，p = 0.03），并且RV 16诱导的IFN-γ：IL-5的比率与%预测FEV 1相关（r = 0.53，p = 0.02）。与此相反，哮喘严重程度的测量和RV诱导的IL-5之间没有显着的关联。这些研究结果表明，细胞因子失衡与RV的Th 1反应不足，但不是Th 2反应，与哮喘严重程度的措施，并支持抗病毒反应受损可能与哮喘严重程度的概念。
The majority of asthma exacerbations are caused by respiratory infections, with rhinovirus (RV) being the most common virus. Recent evidence has suggested that decreased generation of IFN-gamma is associated with more severe colds and delayed elimination of virus. Whether the generation of IFN-gamma, also has any relationship to general features of asthma severity has yet to be determined. To evaluate this hypothesis, peripheral blood mononuclear cells from 19 subjects with atopy and asthma were incubated with RV16 for 6 days to determine IFN-gamma and interleukin (IL)-5 production; these responses were then compared with measurements of airflow obstruction and airway responsiveness. RV16-induced IFN-gamma production correlated significantly with the methacholine PD (r = 0.50, p = 0.03), and the ratio of RV16-induced IFN-gamma:IL-5 correlated with % predicted FEV1 (r = 0.53, p = 0.02). In contrast, there were no significant associations between measures of asthma severity and RV-induced IL-5. These findings suggest that a cytokine imbalance with a deficient Th1 response to RV, but not a Th2 response, is associated with measures of asthma severity and support the concept that impaired antiviral responses may be associated with asthma severity.