Genetic Analysis of Recombination-Deficient Mutants of Escherichia coli K-12 Carrying rec Mutations Cotransducible with thyA

Genetic Analysis of Recombination-Deficient Mutants of Escherichia coli K-12 Carrying rec Mutations Cotransducible with thyA
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携带可与 thyA 共转导的 rec 突变的大肠杆菌 K-12 重组缺陷突变体的遗传分析

DOI:
10.1128/jb.100.2.923-934.1969
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发表时间:
1969
影响因子:
3.2
通讯作者:
D. Mount
D. Mount
中科院分区:
生物学3区
文献类型:
--
作者:
N. Willetts;D. Mount

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20株大肠杆菌K-12携带的rec突变位于thyA附近,这些菌株具有遗传重组缺陷,对紫外线和X射线敏感,其λ溶原菌显示自发噬菌体产生。在其中15个菌株中,rec突变未能补充recB 21,但补充了rec-22。其他5个菌株携带rec突变,其与recB 21互补,而不是rec-22。这些突变比那些不能互补recB 21的突变更接近thyA。因此,它们似乎在不同的重组基因中有缺陷,表示为recC。recB和recC在E. coliK-12中的重组子为thyA-recC-recB-argA。
The rec mutations carried by 20 strains of Escherichia coli K-12 which are defective in genetic recombination and sensitive to ultraviolet light and X rays, and whose λ lysogens show spontaneous phage production, have been mapped near thyA. In 15 of the strains, the rec mutation fails to complement recB21 but complements rec-22. The other five strains carry a rec mutation which complements recB21 but not rec-22. These mutations map closer to thyA than those which fail to complement recB21. They therefore appear to be defective in a different recombination gene, denoted recC. The order of recB and recC on the linkage map of E. coli K-12 is thyA-recC-recB-argA.
DOI: --
发表时间: 1966-06
期刊: Genetics
影响因子: 3.3
作者:
P. Howard-Flanders;L. Theriot
通讯作者: P. Howard-Flanders;L. Theriot