Differences of Ca2+ regulation in skin fibroblasts from blacks and whites.
Differences of Ca2+ regulation in skin fibroblasts from blacks and whites.
复制标题
黑人和白人皮肤成纤维细胞中 Ca2+ 调节的差异。
DOI：
10.1002/jcp.1041380220
复制
发表时间：
1989
影响因子：
5.6
通讯作者：
Aviv,A
中科院分区：
文献类型：
--
作者：
Nakamura,A;Gardner,J;Hatori,N;Nakamura,M;Fine,BP;Aviv,A
黑人比白种人更容易患原发性高血压。为了探讨这种种族差异与细胞Ca 2+代谢有关的可能性，我们测量了连续传代的血压正常的黑人和白色男性皮肤成纤维细胞中45 Ca 2+的洗脱和摄取以及胞浆游离Ca 2 +[Ca 2 +]浓度。根据实验条件，这些细胞中的45 Ca 2+洗脱由两个或三个指数函数描述，而45 Ca 2+摄取仅由两个指数函数描述。未受刺激的成纤维细胞45 Ca ~（2+）摄取和清除无种族差异。然而，人血清刺激导致45 Ca 2+洗脱增加，黑人成纤维细胞的45 Ca 2+洗脱高于白人。种族差异主要表现为从最大和最快可交换的细胞池中45 Ca 2+的表观洗脱速率常数（k1）较高。人血清的作用与其来源无关（黑人vs.白人）。在2 mM Ca 2+培养基和10%黑人血清中，（平均值± SEM; × 10−2/min）黑人和白人成纤维细胞的值分别为89.68 ± 5.23和73.29 ± 4.0;在10%白人血清存在下，黑人和白人细胞的k1值分别为84.14 ± 2.80和76.36 ± 3.23（总体显著性P 0.01）。在含10%人血清的Ca 2+缺乏培养基中，黑人和白人成纤维细胞的k1分别为115.57 ± 3.76和102.15 ± 3.30（P <0.05）。血清显著增加了黑人和白人成纤维细胞对45 Ca 2+的摄取;然而，没有观察到种族差异。[Ca 2 +]的基础水平在黑人与白人的成纤维细胞中没有差异（黑人和白人分别为46.8 ± 6.8和43.2 ± 7.1 nM）。然而，对于5%人血清刺激的细胞，黑人中Ca 2+瞬变的峰值响应显著高于白人（黑人= 963 ± 213，白人= 481 ± 162 nM; P = 0.0286）。我们得出结论，在血清刺激的黑人和白人成纤维细胞中，Ca 2+调节是不同的，至少部分地，这种差异可能与黑人成纤维细胞中更大的激动剂诱导的Ca 2+动员有关。
Black people have a higher propensity than caucasions toward essential hypertension. To explore the possibility that this racial difference relates to cellular Ca2+metabolism, we measured45Ca2+washout and uptake and cytosolic free concentration of Ca2+[Ca2+], in serially passed skin fibroblasts from normotensive black and white males. Depending on the experimental conditions,45Ca2+washout in these cells was described by either two or three exponential functions, whereas45Ca2+uptake was described only by a two‐exponent function. There were no racial differences in45Ca2+uptake and washout of unstimulated fibroblasts. However, stimulation by human serum resulted in an increase in the45Ca2+washout that was higher in fibroblasts from blacks than from whites. The racial differences were expressed primarily by higher values of the apparent washout rate constant (k1) of45Ca2+from the largest and most rapidly exchangeable cellular pool. The effect of human serum was not related to its origin (blacks vs. whites). In 2 mM Ca2+medium and 10% serum from blacks, the respective k1(mean ± SEM; × 10−2/min) values for fibroblasts from blacks and whites were 89.68 ± 5.23 and 73.29 ± 4.0; in the presence of 10% serum from whites, the k1values for cells from blacks and whites were 84.14 ± 2.80 and 76.36 ± 3.23 (overall significance of P .01). In Ca2+‐deficient medium in the presence of 10% human serum, the k1for fibroblasts from blacks and whites were 115.57 ± 3.76 and 102.15 ± 3.30 (P < .05). Serum substantially increased the45Ca2+uptake in fibroblasts from both blacks and whites; however, racial differences were not observed. Basal levels of [Ca2+], were not different in fibroblasts of blacks vs. whites (46.8 ± 6.8 and 43.2 ± 7.1 nM for blacks and whites, respectively). However, the peak response of Ca2+transients for cell stimulated by 5% human serum was significantly higher in blacks than whites (blacks = 963 ± 213, whites = 481 ± 162 nM; P = .0286). We conclude that Ca2+regulation is different in serum‐stimulated fibroblasts from blacks and whites and that, at least in part, this difference may relate to a greater agonist‐induced mobilization of Ca2+in fibroblasts from blacks.