DETECTION OF AN IMMEDIATE EARLY HERPES-SIMPLEX VIRUS TYPE-1 POLYPEPTIDE IN TRIGEMINAL GANGLIA FROM LATENTLY INFECTED ANIMALS
DETECTION OF AN IMMEDIATE EARLY HERPES-SIMPLEX VIRUS TYPE-1 POLYPEPTIDE IN TRIGEMINAL GANGLIA FROM LATENTLY INFECTED ANIMALS
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DOI：
10.1128/iai.34.3.987-992.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
DUNKEL, EC
中科院分区：
文献类型：
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作者：
GREEN, MT;COURTNEY, RJ;DUNKEL, EC
将急性 1 型单纯疱疹病毒 (HSV-1) 感染动物的三叉神经感觉神经节与潜伏感染动物的三叉神经感觉神经节的 HSV 特异性抗原的表达进行比较。通过间接免疫荧光测定，使用针对MW 175,000的立即早期多肽（命名为VP175或ICP4）的抗血清和针对HSV-1的超免疫抗血清来确定早期病毒多肽是否在感染潜伏阶段在神经元中表达。当用抗 HSV-1 或抗 VP175 处理时，来自急性感染动物（感染后 3-12 天处死）的所有 17 个神经节均表现出阳性染色。在感染潜伏期（感染后 22-200 天）处死的动物的 42 个神经节中，有 40 个在用抗 VP175 处理时表现出免疫荧光染色。染色似乎与用抗VP175染色的急性感染动物的神经节中观察到的相似，只是染色细胞的数量和分布显着减少。当用抗-VP175或抗-HSV-1染色时，或者当来自潜伏感染动物的神经节用抗-HSV-1或免疫前血清染色时，未在未感染对照动物的神经节中观察到免疫荧光。
Trigeminal sensory ganglia from animals with acute herpes simplex virus type 1 (HSV-1) infection were compared to those with a latent infection for the expression of HSV-specific antigens. By the indirect immunofluorescence assay, antisera to an immediate early polypeptide of MW 175,000, designated VP175 or ICP4, and a hyperimmune antiserum to HSV-1 were used to determine whether early viral polypeptides were being expressed in neurons during the latent stage of infection. All 17 ganglia from animals with acute infection (sacrificed 3-12 days postinfection) exhibited positive staining when treated either with anti-HSV-1 or with anti-VP175. Forty of 42 ganglia from animals sacrificed during the latent stage of infection (22-200 days postinfection) exhibited immunofluorescent staining when treated with anti-VP175. The staining appeared to be similar to that observed in ganglia from acutely infected animals stained with anti-VP175 except that the number and distribution of stained cells were markedly reduced. No immunofluorescence was observed in ganglia from noninfected control animals when stained with anti-VP175 or anti-HSV-1, or when ganglia from latently infected animals were stained with anti-HSV-1 or preimmune serum.