Molecular genetics of Porphyromonas gingivalis: gingipains and other virulence factors.
Molecular genetics of Porphyromonas gingivalis: gingipains and other virulence factors.
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DOI：
10.2174/1389203033486983
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发表时间：
2003-11
影响因子：
2.8
通讯作者：
K. Nakayama
中科院分区：
文献类型：
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作者：
K. Nakayama
牙龈卟啉单胞菌是一种黑色厌氧革兰氏阴性细菌，是成人慢性牙周炎的主要病原体，是一种牙齿支持组织的炎症性疾病。牙龈假单胞菌具有多种潜在的毒力因子。其中，细胞表面相关和分泌的蛋白酶，如精氨酸和赖氨酸，因其能降解多种宿主蛋白而引起炎症而备受关注。分子遗传学分析在评估病原微生物中每个毒力因子的重要性方面是极其强大的。本文将介绍分子遗传学在牙龈假单胞菌致病机理分析中的应用，以及利用各种潜在毒力因子，特别是蛋白水解酶的敲除突变体所获得的研究结果。
Porphyromonas gingivalis is a black-pigmented anaerobic gram-negative bacterium that is a major pathogen of chronic adult periodontitis, an inflammatory disease of tooth-supporting tissues. P. gingivalis possesses a number of potential virulence factors. Among them, cell-surface-associated and secreted proteinases such as Arg-gingipain and Lys-gingipain have received much attention because they can degrade various host proteins and cause inflammation. Molecular genetic analysis is extremely powerful to evaluate the significance of each virulence factor in a pathogenic microorganism. This review will describe the introduction of molecular genetics to analysis of pathogenesis of P. gingivalis and the findings that have been obtained using knockout mutants of various potential virulence factors, especially proteinases.