N-formyl-methionyl-leucyl-phenylalanine induces and modulates IL-1 and IL-6 in human PBMC.
N-formyl-methionyl-leucyl-phenylalanine induces and modulates IL-1 and IL-6 in human PBMC.
复制标题
N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸诱导和调节人 PBMC 中的 IL-1 和 IL-6。
DOI：
10.1006/cyto.1996.0063
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发表时间：
1996
期刊：
影响因子：
3.8
通讯作者：
Daniel Oth
中科院分区：
文献类型：
--
作者：
Nathalie Arbour;Pierre Tremblay;Daniel Oth
N-甲酰-甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）是一种细菌衍生物，可诱导和调节与炎症相关的各种细胞反应。在这项工作中，我们评估了fMLP刺激对三种促炎细胞因子的影响：IL-1 α，IL-1 β和IL-6。我们发现fMLP诱导人外周血单个核细胞（PBMC）分泌IL-1 α、IL-1 β和IL-6。它还增加了LPS诱导的这三种细胞因子的分泌。北方印迹分析表明，fMLP诱导人PBMC表达IL-1 α、IL-1 β和IL-6基因。百日咳毒素预处理可抑制fMLP诱导的IL-1 α和IL-1 β基因表达及IL-6分泌，提示fMLP诱导的细胞因子也是通过Gi蛋白介导的。用于以剂量依赖性方式获得这些效果的fMLP的浓度范围为20 μ M至1100 μ M。fMLP调节细胞因子分泌的机制仍不清楚。fMLP似乎与能够调节细胞因子的其他趋化因子（C5 α、MCP-1、PAF、IL-8）共享相似的生物活性，并且其受体与fMLP受体属于相同的超家族。
N-formyl-methionyl-leucyl-phenylalanine (fMLP), a bacterial derivative, induces and modulates various cellular responses linked to inflammation. In this work we evaluated the impact of fMLP stimulation on three pro-inflammatory cytokines: IL-1 alpha, IL-1 beta and IL-6. We found that fMLP induces the secretion of IL-1 alpha, IL-1 beta and IL-6 in human peripheral blood mononuclear cells (PBMC). It also increased LPS-induced secretion of these three cytokines. Northern blot analysis demonstrated that fMLP induced IL-1 alpha, IL-1 beta and IL-6 gene expression by human PBMC. The fMLP-induced IL-1 alpha and IL-1 beta gene expression and IL-6 secretion were abolished by pertussis toxin pretreatment, which suggests that the fMLP induction of cytokine was also mediated via a Gi protein. The concentration range of fMLP used to obtain these effects, in a dose dependent fashion, was 20 microM to 1100 microM. The mechanism by which fMLP modulates cytokine secretion is still not characterized. fMLP seems to share similar biological activities with other chemotactic factors (C5a, MCP-1, PAF, IL-8) that are able to modulate cytokines, and whose receptors belong to the same superfamily as the fMLP receptor(s).