Glutamatergic Projections from the Posterior Complex of the Anterior Olfactory Nucleus to the Amygdala Complexes
Glutamatergic Projections from the Posterior Complex of the Anterior Olfactory Nucleus to the Amygdala Complexes
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DOI：
10.1016/j.neuroscience.2023.04.024
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发表时间：
2023-05-11
期刊：
影响因子：
3.3
通讯作者：
Takeuchi，Yukari
中科院分区：
文献类型：
--
作者：
Lu，Ming-Hsuan;Uematsu，Akira;Takeuchi，Yukari
社会缓冲是一种现象，在这种现象中，压力反应被一个从属的同构者所改善。我们之前的研究结果表明，前嗅核（AOP）的后复合体很好地参与了社会缓冲的神经机制。然而，缺乏解剖信息使我们无法进一步估计AOP的作用。在这里，我们获得了关于雄性大鼠AOP的解剖学信息。实验1 （n= 5）中，在AOP中4′，6-二氨基-2-苯基吲哚阳性细胞中，谷氨酸脱羧酶67 （GAD67）阳性细胞比例为13.8%±1.2%。在实验2 （n= 5）中，通过向杏仁核基底外侧复合体（BLA）注射逆行示踪剂标记的细胞中，gad67阳性细胞的比例为18.6%±0.8%。在实验3 （n= 5）中，我们证明了通过注射到内侧杏仁核（MeP）后部的逆行示踪剂标记的细胞的存在，主要是在MeP的腹侧部分。此外，gad67阳性细胞占示踪剂标记细胞的比例为21.7%±1.7%。在实验4 （n= 3）中，逆行示踪剂注射到BLA和MeP中，主要注射到MeP的腹侧。双标记细胞占示踪剂标记细胞的比例为2.1%±1.2%。综上所述，这些结果表明AOP主要由谷氨酸能神经元组成。此外，AOP向BLA和MeP发送相互独立的以谷氨酸为主的投射。
Social buffering is a phenomenon where stress responses are ameliorated by an affiliative conspecific. Our previous findings suggest that the posterior complex of the anterior olfactory nucleus (AOP) is well positioned to participate in the neural mechanisms underlying social buffering. However, the lack of anatomical information prevents us from further estimating the role of the AOP. Here, we obtained anatomical information regarding the AOP in male rats. In Experiment 1 (n= 5), among 4′,6-diamidino-2-phenylindole-positive cells in the AOP, the proportion of glutamic acid decarboxylase 67 (GAD67)-positive cells was 13.8% ± 1.2%. In Experiment 2 (n= 5), among the cells that were labeled by a retrograde tracer injected into the basolateral complex of the amygdala (BLA), the proportion of GAD67-positive cells was 18.6% ± 0.8%. In Experiment 3 (n= 5), we demonstrated the existence of cells that were labeled by the retrograde tracer injected into the posterior part of the medial amygdala (MeP), mostly into the ventral part of the MeP. In addition, the proportion of GAD67-positive cells among the tracer-labeled cells was 21.7% ± 1.7%. In Experiment 4 (n= 3), the retrograde tracers were injected into the BLA and MeP, mostly into the ventral part of the MeP. The proportion of double-labeled cells among the tracer-labeled cells was 2.1% ± 1.2%. Taken together, these results suggest that the AOP is predominantly composed of glutamatergic neurons. In addition, the AOP sends mutually independent glutamatergic-predominant projections to the BLA and MeP.