Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs.
Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs.
复制标题
DOI：
10.1016/j.ydbio.2017.04.008
复制
发表时间：
2017-06-01
影响因子：
2.7
通讯作者：
Newmark PA
中科院分区：
文献类型：
--
作者：
Rouhana L;Tasaki J;Saberi A;Newmark PA
胞质多聚腺苷酸化是后生动物生殖细胞中普遍存在的mRNA调节机制;它在很大程度上依赖于胞质多聚腺苷酸化元件结合蛋白（CPEBs）。两个CPEB同源物中确定的Planarian Schmidtea mediterranea。Smed-CPEB 1在性成熟的真涡虫卵巢和卵黄腺中表达，是卵母细胞和卵黄腺发育所必需的。相比之下，Smed-CPEB 2在睾丸和中枢神经系统中表达;其功能是精子发生所需的，也是卵巢和辅助生殖器官发育所需的非自主功能。CPEB基因敲除动物的转录组分析揭示了一个全面的收集的分子标记的生殖结构在S。包括卵巢、睾丸、卵黄腺和交配器。RNA干扰分析揭示了这些基因中的十几个在卵子发生、精子发生或包囊形成过程中的作用。我们还提出的证据表明，Smed-CPEB 2促进翻译的神经肽Y-8，所需的涡虫性成熟激素原。这些研究结果提供了潜在的生殖细胞发育的保守过程，以及参与胶囊沉积扁形虫的事件的机制的见解。
Cytoplasmic polyadenylation is a mechanism of mRNA regulation prevalent in metazoan germ cells; it is largely dependent on Cytoplasmic Polyadenylation Element Binding proteins (CPEBs). Two CPEB homologs were identified in the planarian Schmidtea mediterranea. Smed-CPEB1 is expressed in ovaries and yolk glands of sexually mature planarians, and required for oocyte and yolk gland development. In contrast, Smed-CPEB2 is expressed in the testes and the central nervous system; its function is required for spermatogenesis as well as non-autonomously for development of ovaries and accessory reproductive organs. Transcriptome analysis of CPEB knockdown animals uncovered a comprehensive collection of molecular markers for reproductive structures in S. mediterranea, including ovaries, testes, yolk glands, and the copulatory apparatus. Analysis by RNA interference revealed contributions for a dozen of these genes during oogenesis, spermatogenesis, or capsule formation. We also present evidence suggesting that Smed-CPEB2 promotes translation of Neuropeptide Y-8, a prohormone required for planarian sexual maturation. These findings provide mechanistic insight into potentially conserved processes of germ cell development, as well as events involved in capsule deposition by flatworms.
登录
查看更多内容
影响因子：
11.4
作者：
Huang, Yi-Shuian;Kan, Ming-Chung;Richter, Joel D.
通讯作者：
Richter, Joel D.
影响因子：
5.3
作者：
Kan, Ming-Chung;Oruganty-Das, Aparna;Richter, Joel D.
通讯作者：
Richter, Joel D.
影响因子：
2.6
作者：
ISHIDA, S;TESHIROGI, W
通讯作者：
TESHIROGI, W
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
64.5
作者：
HAKE, LE;RICHTER, JD
通讯作者：
RICHTER, JD