Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise
Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise
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DOI：
10.1152/jappl.1996.81.3.1213
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发表时间：
1996-09-01
影响因子：
3.3
通讯作者：
Yamaya, K
中科院分区：
文献类型：
--
作者：
Suzuki, K;Sato, H;Yamaya, K
为了探讨运动对循环中性粒细胞产生活性氧（ROS）能力的影响的大量文献中存在分歧的原因，10名男性运动员接受了最大限度的运动训练。超氧自由基（O-2（-）.）通过直接在新鲜的未分离的血液上进行的组织化学硝基蓝四唑试验，首先在逐个细胞的基础上检测嗜中性粒细胞，这表明在几种刺激条件下的反应性嗜中性粒细胞在运动后相对减少。类似地，O--（2）.用双-N-甲基吖啶硝酸盐（光泽精）依赖的化学发光（CL）检测的固定数量的纯化的中性粒细胞在用调理剂化的酵母聚糖刺激时的活性在运动后略微降低。与此相反，5-氨基-2，3-二氢-1，4-酞嗪二酮（鲁米诺）依赖CL反应的中性粒细胞指示髓过氧化物酶（MPO）介导的高活性氧化剂的形成显着增强运动后。提示中性粒细胞活性氧代谢途径可能是由前体O-2（-）前向的。由于MPO脱颗粒的促进，生产进入更活性的氧化剂形成的阶段。此外，这些现象与运动引起的中性粒细胞从边缘池进入循环的动员密切相关，这是由运动期间儿茶酚胺的过度分泌介导的。这些发现表明，使用不同的技术来检测ROS或中性粒细胞ROS代谢的不同阶段可以解释以前的研究中的一些不同的结果。
To investigate the cause of disagreement within the large body of literature concerning the effect of exercise on the capacity of circulating neutrophils to produce reactive oxygen species (ROS), 10 male endurance-trained athletes underwent maximal exercise. The generation of superoxide radical (O-2(-).) by neutrophils was first detected on a cell-by-cell basis by using histochemical nitro blue tetrazolium tests performed directly on fresh unseparated blood, which showed that responsive neutrophils under several stimulatory conditions relatively decreased after exercise. Similarly, O--(2). detected with bis-N-methylacridinium nitrate (lucigenin)-dependent chemiluminescence (CL) of a fixed number of purified neutrophils on stimulation with opsonized zymosan was decreased slightly after exercise. In contrast, the 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol)dependent CL response of the neutrophils indicative of the myeloperoxidase (MPO)-mediated formation of highly reactive oxidants was significantly enhanced after exercise. It therefore suggests that the pathway of neutrophil ROS metabolism might be forwarded from the precursor O-2(-). production to the stages of more reactive oxidant formation due to the facilitation of MPO degranulation. In addition, these phenomena were closely associated with the exercise-induced mobilization of neutrophils from the marginated pool into the circulation, which was mediated by the overshooting of catecholamines during exercise. These findings indicate that the use of different techniques for detecting ROS or the different stages of neutrophil ROS metabolism could explain some of the disparate findings of the previous studies.