In vitro and in vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae
In vitro and in vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae
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DOI：
10.1016/j.jep.2008.06.016
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发表时间：
2008-09-02
影响因子：
5.4
通讯作者：
Cho, Jae Youl
中科院分区：
文献类型：
--
作者：
Byeon, Se Eun;Chung, Joo Young;Cho, Jae Youl
研究目的：Tabebuia spp.原产于中美洲和南美洲的热带雨林，长期以来一直被用作治疗细菌感染和凝血的民间药物。癌症和炎症性疾病。材料和方法：采用脂多糖刺激的巨噬细胞和花生四烯酸或巴豆油诱导的小鼠耳肿胀模型。结果：在脂多糖(LPS)刺激的RAW264.7细胞中，非洲茶多酚水提物(Taheebo)显著抑制前列腺素(PG)E-2和一氧化氮(NO)的产生，并阻断其催化酶(环氧合酶[COX)-II]和诱导型一氧化氮合酶(NOS)的mRNA表达。根据免疫印迹分析和NO测定，Taheebo阻断炎症介质似乎是细胞外信号相关激酶(ERK)激活中断的结果，其中LPS强烈诱导ERK的磷酸化(激活的标志)，选择性ERK抑制剂U0126强烈抑制PGE(2)的产生。同样，口服Taheebo(100 mg/kg)1周可完全减轻COX-II激活剂花生四烯酸所致的小鼠耳肿胀，但不能减轻脂氧合酶激活剂巴豆油所致的小鼠耳肿胀。结论：Taheebo的民族药理作用可能与其通过抑制PGE(2)的产生而负性调节巨噬细胞介导的炎症反应有关。因此，这种水提物可能被开发为治疗各种炎症性疾病的新药物，如关节炎和动脉硬化。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Aim of study: Tabebuia spp. (Bignoniaceae) are native to tropical rain forests throughout Central and South America and have long been used as a folk medicine to treat bacterial infection, blood coagulation. cancer and inflammatory diseases. In this study, we aimed to demonstrate the ethnopharmacological activity of Tabebuia avellanedae in various in vitro and in vivo inflammatory conditions.Materials and methods: To do this, LPS-stimulated macrophages and arachidonic acid or croton oil-induced mouse ear edema models were employed.Results: The water extract (taheebo) of Tabebuia avellanedae significantly suppressed the production of prostaglandin (PG) E-2 and nitric oxide (NO), and blocked the mRNA expression of their catalyzing enzymes (cyclooxygenase [COX)-II] and inducible NO synthase [iNOS], respectively), in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The blockade of inflammatory mediators by taheebo seemed to be the result of the interruption of extracellular signal-related kinase (ERK) activation, according to immunoblotting analysis and the NO assay, where LPS strongly induced the phosphorylation (a hallmark of activation) of ERK, and U0126, a selective ERK inhibitor, was found to strongly inhibit PGE(2) production. Similarly, oral administration of taheebo (100 mg/kg) for I week completely diminished mouse ear edema induced by arachidonic acid, an activator of COX-II, but not croton oil, an activator of lipoxygenase.Conclusions: These data suggest that the ethnopharmacological action of taheebo maybe due to its negative modulation of macrophage-mediated inflammatory responses by suppressing PGE(2) production. Thus, this water extract may be developed as a new therapeutic remedy for various inflammatory diseases such as arthritis and atherosclerosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.