Protein nitration during defense response in Arabidopsis thaliana
Protein nitration during defense response in Arabidopsis thaliana
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DOI：
10.1002/elps.200800826
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发表时间：
2009-07-01
期刊：
影响因子：
2.9
通讯作者：
Delledonne, Massimo
中科院分区：
文献类型：
--
作者：
Cecconi, Daniela;Orzetti, Stefano;Delledonne, Massimo
一氧化氮和活性氧在植物超敏抗病反应中起着关键作用，蛋白质酪氨酸硝化是二者协同作用的重要机制。到目前为止，这种翻译后修饰的靶蛋白在植物中仍然完全未知。在这项研究中，我们通过1D和2D-western印迹分析来自用无毒细菌病原体（假单胞菌pv.番茄）。我们发现，植物的抗病性反应与重要的细胞过程，如光合作用，糖酵解和硝酸盐同化的蛋白质的硝化的调制。这些发现揭示了一氧化氮和活性氧的信号功能，为研究这种翻译后修饰在植物中的作用铺平了道路。
Nitric oxide and reactive oxygen species play a key role in the plant hypersensitive disease resistance response, and protein tyrosine nitration is emerging as an important mechanism of their co-operative interaction. Up to now, the proteins targeted by this post-translational modification in plants are still totally unknown. in this study, we analyzed for the first time proteins undergoing nitration during the hypersensitive response by analyzing via 1D- and 2D-western blot the protein extracts from Arabidopsis thaliana plants challenged with an avirulent bacterial pathogen (Pseudomonas syringae pv. Tomato). We show that the plant disease resistance response is correlated with a modulation of nitration of proteins involved in important cellular process, such as photosynthesis, glycolysis and nitrate assimilation. These findings shed new light on the signaling functions of nitric oxide and reactive oxygen species, paving the way on studies on the role of this post-translational modification in plants.