Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins.
Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins.
复制标题
麻疹病毒糖蛋白细胞融合后，腺病毒的细胞毒性增强，且无核内扩散。
DOI：
10.1128/jvi.79.3.1911-1917.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Dobbelstein,Matthias
中科院分区：
文献类型：
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作者：
Horn,GermanP;Vongpunsawad,Sompong;Kornmann,Evelyn;Fritz,Barbara;Dittmer,DirkP;Cattaneo,Roberto;Dobbelstein,Matthias
病毒在癌症治疗中的效率通过介导受感染细胞与其邻近细胞融合的蛋白质来增强。据报道，具有复制能力的腺病毒颗粒可以在融合产生的合胞体内的细胞核之间传播。为了评估这一猜想，我们产生了融合腺病毒，表达麻疹病毒的F和H糖蛋白的平衡比例。与非融合病毒相比，病毒显示出增强的细胞毒性，但复制效率基本不变。最值得注意的是，病毒基因组没有通过融合产生的多核细胞传播。因此，腺病毒在合胞体中的复制仍然主要限于最初转导的细胞核。
The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei.