Fed-batch bioprocess development for astaxanthin production by Xanthophyllomyces dendrorhous based on the utilization of Prosopis sp. pods extract
Fed-batch bioprocess development for astaxanthin production by Xanthophyllomyces dendrorhous based on the utilization of Prosopis sp. pods extract
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DOI：
10.1016/j.bej.2020.107844
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发表时间：
2021-01-14
影响因子：
3.9
通讯作者：
Morales-Oyervides, Lourdes
中科院分区：
文献类型：
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作者：
Angel Villegas-Mendez, Miguel;Papadaki, Aikaterini;Morales-Oyervides, Lourdes
这项工作的重点是利用可再生资源作为微生物生产类胡萝卜素的原料，类胡萝卜素是各种工业中有价值的化合物。以主要合成虾青素和β -胡萝卜素的树状黄叶菌为原料。我们提出了两种策略来实现高产量，正确的供氧介质和采用进料批量模式。两个因素有关的介质的氧转移在烧瓶规模（工作体积和搅拌）进行了评估。采用豆科植物豆荚为基础的培养基（MEM）和化学定义的培养基（YM）成功地将虾青素产量与氧传递系数（k(L)a）进行了相关研究。然后使用k(L)a相关性来设定反应器规模的条件。16.86 h(-)(1)的最佳k(L)a足以促进MEM虾青素的产量（1824.13 μ g/L）。在最高评价水平（17.77 h(-1)）下，YM的最佳产量为1656.24 μ g/L。因此，与MEM相比，YM需要更高的氧传递速率来产生类胡萝卜素。因此，虾青素的生产在烧瓶和反应器规模上使用进料批工艺进行了评估。间歇式补料培养达到4465.12 μ g/L（烧瓶）和4130.23 μ g/L（反应器）的生产水平。本研究证明了氧传递与类胡萝卜素产量之间的相关性，以及在料批生物工艺中利用豆科植物原料提高类胡萝卜素产量的潜力。
This work focuses on using renewable sources as feedstock for the microbial production of carotenoids, valuable compounds for various industries. Xanthophyllomyces dendrorhous, which mainly synthesize astaxanthin and beta-carotene, was used for the process. We proposed two strategies to achieve high production yields, the correct oxygen supply to the medium and the adoption of a fed-batch mode. Two factors related to the medium's oxygen transfer at a flask scale (working volume and agitation) were evaluated. Astaxanthin production was successfully correlated with the oxygen transfer coefficient (k(L)a) using a mesquite pods-based medium (MEM) and a chemically defined one (YM). k(L)a correlations were then used to set the conditions at a reactor scale. An optimum k(L)a of 16.86 h(-)(1) was adequate to promote astaxanthin production in MEM (1824.13 mu g/L). Meanwhile, in YM, optimum production (1656.24 mu g/L) was obtained at the highest evaluated levels (17.77 h(-1)). Thus, a higher oxygen transfer rate is required to produce carotenoids in YM compared with MEM. Astaxanthin production was thus evaluated using a fed-batch process at flask and reactor scales. Reached production levels with fed-batch cultivation were 4465.12 mu g/L (flask) and 4130.23 mu g/L (reactor). This study demonstrated the correlation between oxygen transfer and carotenoids' production and the potential utilization of mesquite feedstock in a fedbatch bioprocess to improve carotenoids' yield by X. dendrorhous.