DIFFERENCES IN THE HEPATITIS-C VIRUS GENOTYPES IN DIFFERENT COUNTRIES
DIFFERENCES IN THE HEPATITIS-C VIRUS GENOTYPES IN DIFFERENT COUNTRIES
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DOI：
10.1016/s0168-8278(05)80205-3
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发表时间：
1993-03-01
影响因子：
25.7
通讯作者：
DATE, T
中科院分区：
文献类型：
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作者：
TAKADA, N;TAKASE, S;DATE, T
根据核苷酸序列的不同，我们最近将丙型肝炎病毒（HCV）分为4型（HCV-PT、-K1、-K2 a和-K2 b）。发现从美国报道的原型HCV-PT，在日本是罕见的，这表明HCV基因型的分布可能在不同的国家不同。因此，HCV基因型的患病率在不同的国家进行了比较。HCV的基因分型通过使用针对每种HCV的特异性cDNA探针的狭缝印迹杂交分析或通过限制性片段长度多态性分析进行。在121名日本非癌症患者中，HCV基因型的患病率为HCV-K1 77.7%，HCV-K2 a 16.5%和HCV-K2 b 5.0%。HCV-PT仅在1例患者中检出（0.8%）。43例日本肝细胞癌（HCC）患者中HCV-KI、HCV-K2 a和HCV-K2 b的患病率分别为74.4%、18.6%和4.7%。HCV-PT仅在1例标本中检出。在19例欧洲非癌症患者中，HCV-PT的检出率为42.1%，HCV-K1的检出率为52.6%。所有7例欧洲HCC患者的样本均为HCV-K1，表明其患病率显著高于非癌症患者。在13名巴西非癌症患者中，分布模式与欧洲人相似。在来自美国的10个样本中，HCV-PT检出率为70%，HCV-K2检出率为1。在18例非肿瘤患者中，HCV-K1阳性率为44.4%，HCV-K2 a阳性率为50.0%，HCV-K2 b阳性率为5.6%。未发现HCV-PT，2例中国人肝癌标本为HCV-K1。这些结果表明，HCV基因型的分布模式在不同的国家有很大的差异。除美国外，HCV-K1的流行率在每个国家都很高，提示HCV-KI可能是世界范围内HCV的主要类型。HCV-PT偶尔在日本和中国的样本中发现，但在西方国家的样本中更常见。另一方面，HCV-K2在西方国家的样本中很少发现，但在日本和中国的样本中很常见。这些差异提示HCV-PT可能为西方型，HCV-K2可能为东方型。
We recently classified the hepatitis C virus (HCV) into 4 types (HCV-PT, -K1, -K2a and -K2b) according to differences in nucleotide sequences. it was found that HCV-PT, the prototype reported from the U.S.A., was rare in Japan, suggesting that distribution of HCV genotypes may be different in various countries. The prevalence of HCV genotypes was therefore compared in different countries. Genotyping of HCV was performed by slot-blot hybridization analysis using cDNA probes specific to each type of HCV or by restriction fragment length polymorphism analysis. In 121 Japanese non-cancer patients, the prevalence of HCV genotypes was 77.7% for HCV-K1, 16.5% for HCV-K2a and 5.0% for HCV-K2b. HCV-PT was detected in only 1 patient (0.8%). The prevalence in 43 Japanese hepatocellular carcinoma (HCC) patients was 74.4% for HCV-KI, 18.6% for HCV-K2a and 4.7% for HCV-K2b. HCV-PT was found in only 1 sample. In 19 European non-cancer patients, HCV-PT was found in 42.1% and HCV-K1 was found in 52.6%. HCV-K2 was not found. All 7 samples from European HCC patients were HCV-K1, indicating a significantly higher prevalence than in non-cancer patients. In 13 Brazilian non-cancer patients, the distribution pattern was similar to that of the Europeans. In 10 samples from the U.S.A., HCV-PT was found in 70% and HCV-K2 was found in 1 sample. In 18 Chinese noncancer patients, HCV-K1 was found in 44.4%, HCV-K2a in 50.0% and HCV-K2b in 5.6%. HCV-PT was not found. Two samples from Chinese HCC patients were HCV-K1. These results indicate that the distribution patterns of HCV genotypes vary widely among different countries. Prevalence of HCV-K1 was high in every country except for the U.S.A., suggesting that HCV-KI may be the major type of HCV worldwide. HCV-PT was occasionally found in the Japanese and Chinese samples but was more common in the samples from Western countries. On the other hand, HCV-K2 was rarely found in samples from Western countries but was commonly found in the Japanese and Chinese samples. These differences suggest that HCV-PT may be the Western type and HCV-K2 may be the Oriental type.