3D sample preparation for orthopaedic tissue engineering bioreactors.

3D sample preparation for orthopaedic tissue engineering bioreactors.
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骨科组织工程生物反应器的 3D 样品制备。

DOI:
10.1007/978-1-60761-984-0_5
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发表时间:
2011
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Cartmell SH
Cartmell SH
中科院分区:
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文献类型:
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作者:
Cartmell SH

文献摘要

相似文献

目前有几种类型的生物反应器可用于骨科组织工程结构的培养。这些在设计和文化上从简单到复杂都有不同。生物反应器样品的制备因使用的系统不同而不同。本章介绍了用于骨和韧带组织体外培养的旋转合成生物反应器(Cellon)、平板振荡器、灌流系统和Bose Electrforce Systems生物动力学仪器的3D样品的制备方法,并介绍了经过验证和测试的方法。
There are several types of bioreactors currently available for the culture of orthopaedic tissue engineered constructs. These vary from the simple to the complex in design and culture. Preparation of samples for bioreactors varies depending on the system being used. This chapter presents data and describes tried and tested methodologies for the preparation of 3D samples for a Rotatory Synthecon Bioreactor (Cellon), a plate shaker, a perfusion system, and a Bose Electroforce Systems Biodynamic Instrument for the in vitro culture of bone and ligament tissue.