Sympatric lineages in the Mantidactylus ambreensis complex of Malagasy frogs originated allopatrically rather than by in-situ speciation.
Sympatric lineages in the Mantidactylus ambreensis complex of Malagasy frogs originated allopatrically rather than by in-situ speciation.
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DOI：
10.1016/j.ympev.2019.106700
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发表时间：
2019-12
影响因子：
4.1
通讯作者：
Safidy M Rasolonjatovo;M. Scherz;C. Hutter;F. Glaw;Andolalao Rakotoarison;Jary H. Razafindraibe;S. Goodman-S
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文献类型：
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作者：
Safidy M Rasolonjatovo;M. Scherz;C. Hutter;F. Glaw;Andolalao Rakotoarison;Jary H. Razafindraibe;S. Goodman-S
马达加斯加的生物区系的特点是在不同的分类层次上具有高度的微特有性，但在这个岛上很少研究殖民化和原位物种形成如何有助于当地物种群落的组装。在这里，我们分析的theMantidactylus ambreensis物种复杂，这是分布在马达加斯加北部，最初描述从蒙塔涅德安布尔，一个孤立的火山起源的山，目前保护内蒙塔涅德安布尔国家公园（MANP）的河岸青蛙的系统发育关系。来自线粒体DNA的数据和来自序列捕获方法FrogCap的cDNA基因组数据独立地证实了该物种复合体在Ochthomantis亚属内是单系的，并确定了该亚属内的两个主要分支。这两个分支在线粒体16 S rRNA基因中相隔5.6-6.8%的成对距离，并共同出现在MANP中。一种分布在高海拔地区（海拔940-1375米）。另一个在较低海拔（海拔535-1010米），但在核RAG 1基因中几乎没有单倍型共享。这种在没有混合的同生现象中的发生证实了它们是独立的进化谱系，值得作为单独的物种来认识，我们在这里将它们称为高海拔（HE）和低海拔（LE）谱系;它们将保证分类学评估，以确信地将ambreensis命名为其中之一。人口M。来自马达加斯加北方其他地方的ambreensiscomplex都属于LE谱系，尽管它们确实比Montagne d 'Ambre（海拔285-1040米）的海拔范围更大。在LE内有几个中度深度分歧的E1组（LE 1-LE 4）（16 S中为1.5-2.8%），但MANP中出现的E1组LE 4具有深度嵌套的系统发育位置，如通过线粒体和序列捕获数据集分别恢复的。这表明，HE和LE没有分歧的低和高人口的局部裂变，而是通过一个更复杂的地理情景出现。LE 1-LE 4类群的分枝格局具有明显的南北向的地理格局。从这些结果中，我们得出一个可检验的假设vicariant物种形成，限制了HE谱系MANP和LE候选物种的气候避难所进一步南部，随后向北范围扩大和二次殖民MANP LE。这些结果提供了一个例子，复杂的组装当地微型特有的两栖动物区系马达加斯加。
Madagascar's biota is characterized by a high degree of microendemism at different taxonomic levels, but how colonization and in-situ speciation contribute to the assembly of local species communities has rarely been studied on this island. Here we analyze the phylogenetic relationships of riparian frogs of theMantidactylus ambreensisspecies complex, which is distributed in the north of Madagascar and was originally described from Montagne d’Ambre, an isolated mountain of volcanic origin, currently protected within Montagne d'Ambre National Park (MANP). Data from mitochondrial DNA, and phylogenomic data from FrogCap, a sequence capture method, independently confirm that this species complex is monophyletic within the subgenusOchthomantis, and identify two main clades within it. These two clades are separated by 5.6–6.8% pairwise distance in the mitochondrial 16S rRNA gene and co-occur in MANP, with one distributed at high elevations (940–1375 m a.s.l.) and the other at lower elevations (535–1010 m a.s.l.), but show almost no haplotype sharing in the nuclear RAG1 gene. This occurrence in syntopy without admixture confirms them as independent evolutionary lineages that merit recognition as separate species, and we here refer to them as high-elevation (HE) and low-elevation (LE) lineage; they will warrant taxonomic assessment to confidently assign the nameambreensisto one or the other. Populations of theM. ambreensiscomplex from elsewhere in northern Madagascar all belong to the LE lineage, although they do occur over a larger elevational range than in Montagne d'Ambre (285–1040 m a.s.l.). Within LE there are several phylogroups (LE1–LE4) of moderately deep divergence (1.5–2.8% in 16S), but phylogroup LE4 that occurs in MANP has a deeply nested phylogenetic position, as recovered separately by mitochondrial and sequence capture datasets. This suggests that HE and LE did not diverge by a local fission of lower and upper populations, but instead arose through a more complex biogeographic scenario. The branching pattern of phylogroups LE1–LE4 shows a clear south-to-north phylogeographic pattern. We derive from these results a testable hypothesis of vicariant speciation that restricted the HE lineage to MANP and the LE candidate species to a climatic refugium further south, with subsequent northwards range expansion and secondary colonization of MANP by LE. These results provide an example for complex assembly of local microendemic amphibian faunas on Madagascar.