Infectious bronchitis viruses with naturally occurring genomic rearrangement and gene deletion
Infectious bronchitis viruses with naturally occurring genomic rearrangement and gene deletion
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DOI：
10.1007/s00705-010-0850-6
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Noormohammadi, Amir H.
中科院分区：
文献类型：
--
作者：
Hewson, Kylie A.;Ignjatovic, Jagoda;Noormohammadi, Amir H.
传染性支气管炎病毒（IBV）是感染全世界家禽的III组冠状病毒。包括全基因缺失在内的遗传变异是IBV进化的关键。澳大利亚亚组2 IBV含有序列插入和多个基因缺失，导致与国际IBV的基因组差异很大。研究了澳大利亚IBV中存在的基因组变异，并与另一种III组冠状病毒火鸡冠状病毒（TCoV）进行了比较。在澳大利亚IBV基因组中发现的开放阅读框（ORF）在序列和位置上与TCoV ORF相似，但ORF 4b除外，其似乎在亚组2菌株中易位到不同的位置。亚群2菌株先前报道缺乏基因3a、3b和5a，有些还缺乏5b。然而，在这些基因中，发现基因3b和5b存在，但含有可能影响转录的各种突变。在这项研究中，发现亚组2 IBV经历了比以前认为的更实质性的基因组重排。
Infectious bronchitis viruses (IBVs) are group III coronaviruses that infect poultry worldwide. Genetic variations, including whole-gene deletions, are key to IBV evolution. Australian subgroup 2 IBVs contain sequence insertions and multiple gene deletions that have resulted in a substantial genomic divergence from international IBVs. The genomic variations present in Australian IBVs were investigated and compared to those of another group III coronavirus, turkey coronavirus (TCoV). Open reading frames (ORFs) found throughout the genome of Australian IBVs were analogous in sequence and position to TCoV ORFs, except for ORF 4b, which appeared to be translocated to a different position in the subgroup 2 strains. Subgroup 2 strains were previously reported to lack genes 3a, 3b and 5a, with some also lacking 5b. Of these, however, genes 3b and 5b were found to be present but contained various mutations that may affect transcription. In this study, it was found that subgroup 2 IBVs have undergone a more substantial genomic rearrangements than previously thought.