Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea
Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea
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DOI：
10.1007/s00436-010-2101-x
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Kim, Jeong-Ho
中科院分区：
文献类型：
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作者：
Setyobudi, Eko;Jeon, Chan-Hyeok;Kim, Jeong-Ho
调查了韩国东海岸南大河的大麻哈鱼（Oncorhynchus凯塔）的感染率和异尖线虫幼虫的鉴定。2008年，从120个鱼类样本（雄性= 58条鱼，雌性= 62条鱼）中总共收集了8，358条幼虫。2008年10月至11月期间采集了鱼类样本。捕获的所有鲑鱼样本（120/120，100%）均感染了高强度的异尖线虫幼虫（69.65 +/- 48.58幼虫/宿主）。主要分布在肌肉中（98.00%）。根据形态学、PCR-RFLP分子分析和线粒体DNA cox 2基因标记测序，这些线虫被鉴定为单纯异尖线虫（狭义）第三期幼虫。这是第一份关于韩国鲑科鱼类异尖蠕虫分子鉴定的报告。虽然大麻哈鱼的商业价值相对低于其他鲑鱼科物种，但如果生食或未煮熟，大麻哈鱼中异尖蠕虫的高发生率可能会造成相当大的食品安全问题。
The prevalence of infection and the identification of anisakid larvae in chum salmon (Oncorhynchus keta) from the Namdae River, the east coast of Korea, were investigated. In total, 8,358 larvae were collected from 120 fish samples (male = 58 fish, female = 62 fish) in 2008. Fish samples were collected during October and November 2008. All the chum salmon samples (120/120, 100%) caught were infected with anisakid larvae with a high intensity (69.65 +/- 48.58 larvae/host). They were mostly found in muscles (98.00%). Based on the morphological and the molecular analysis of PCR-RFLP and sequencing of mitochondrial DNA cox2 gene markers, these nematodes were identified as Anisakis simplex (sensu stricto) third-stage larvae. This is the first report on the molecular identification of anisakid worms from salmonid fishes in Korea. The high occurrence of anisakid worms in chum salmon may pose considerable food safety problems if they were consumed as raw or undercooked, although their commercial value is relatively lower than other salmonid species.