Chitosan monomer promotes tissue regeneration on dental pulp wounds
Chitosan monomer promotes tissue regeneration on dental pulp wounds
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DOI：
10.1002/jbm.a.30588
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发表时间：
2006-03-15
影响因子：
4.9
通讯作者：
Hayashi, Y
中科院分区：
文献类型：
--
作者：
Matsunaga, T;Yanagiguchi, K;Hayashi, Y
本研究对壳聚糖单体（盐酸d -氨基葡萄糖）作为纸浆封盖剂的适用性进行了评价。通过体外和体内实验，研究壳单糖对大鼠创伤后细胞代谢和创面愈合机制的影响。在成骨细胞培养3 d后，壳聚糖组碱性磷酸酶（ALP）活性显著升高。逆转录聚合酶链反应分析显示，壳聚糖诱导成骨细胞培养3 d后ALP mRNA表达增加，培养7 d后骨形态发生蛋白-2 mRNA表达增加。在添加壳聚糖单体的培养基中，成纤维细胞的炎性细胞因子白介素-8的合成受到强烈抑制。在大鼠实验中评价组织病理学效应。实验结束后，与壳聚糖聚合物相比，炎症细胞浸润较弱。3天后，在壳聚糖单体附近观察到成纤维细胞显著增殖。炎性细胞浸润几乎完全消失。5 d后，成纤维细胞增殖进展，增生成纤维细胞周围出现成牙细胞。这些结果表明，与壳聚糖聚合物相比，本研究首次报道了壳聚糖单体在伤口愈合初期具有生物相容性稳定的作用。(c) 2005 Wiley期刊有限公司
The present study was undertaken to evaluate the applicability of chitosan monomer (D-glucosamine hydrochloride) as a Pulp capping medicament. Both hi vitro and in vivo experiments were carried out to study the cell metabolism and Wound healing mechanisms following the application of chitomonosaccharide. After 3 days of osteoblast culture, alkaline phosphatase (ALP) activity Significantly increased in the chitosan group. Reverse transcription polymerase chain reaction analysis revealed that chitosan induced an increase in the expression of ALP mRNA after 3 days and bone morphogenetic protein-2 mRNA after 7 days of osteoblast incubation. Inflammatory cytokine, interleukin (IL)-8, synthesis in fibroblasts was strongly suppressed in the medium Supplemented with chitosan monomer. Histopathological effects were evaluated in rat experiments. After day, inflammatory cell infiltrations were observed to be weak when compared with the application of chitosan polymer. After 3 days, a remarkable proliferation of fibroblasts was seen near the applied chitosan monomer. The inflammatory cell infiltration had almost completely disappeared. After 5 days, the fibroblastic proliferation progressed, and some odontoblastic cells appeared at the periphery of the proliferated fibroblasts. These findings indicate that the present study is the first report that chitosan monomer acts as a biocompatibly stable medicament even at the initial Stage Of wound healing in comparison with the application of chitosan polymer. (c) 2005 Wiley Periodicals, Inc.