Optimization of Reaction Parameters for the Synthesis of Chromium Methionine Complex Using Response Surface Methodology
Optimization of Reaction Parameters for the Synthesis of Chromium Methionine Complex Using Response Surface Methodology
复制标题
DOI：
10.1021/op3002905
复制
发表时间：
2013-04-01
影响因子：
3.4
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
--
作者：
Tang, Hai-yan;Xiao, Qing-gui;Zhang, Yi
本文研究了以乙醇水溶液为反应介质制备蛋氨酸铬的新工艺。采用响应面法（RSM）结合Box-Behnken设计对该方法的操作参数进行建模和优化。乙醇水溶液中的乙醇含量，蛋氨酸和Cr（III）的摩尔比，反应温度，和反应时间被选为自变量，和它们的综合影响的Cr（III）的转化率（响应1）和蛋氨酸铬的收率（响应2）进行了研究。实验结果表明：乙醇水溶液中乙醇含量为48.5%，n（Met）/n（NaOH）/n（CrCl 3中心点6 H（2）O）= 4.31：4.31：1，反应时间为127 min，反应温度为81.1 ℃，Cr（III）初始浓度为0.25 mol/L。在最佳条件下，Cr（III）的转化率和蛋氨酸铬的收率分别达到99.79%和99.23%。方差分析（ANOVA）和回归分析表明，Cr（III）转化率的R-2值为0.9984，蛋氨酸铬收率的R-2值为0.9974，实验结果与预测值吻合较好，该模型可成功地用于蛋氨酸铬合成的预测。
This study deals with a new and better protocol to prepare chromium methionine using aqueous ethanol solution as the reaction medium. Response surface methodology (RSM) coupled with Box-Behnken design was employed to model and optimize the operational parameters of this protocol. The ethanol content of aqueous ethanol, the molar ratio of methionine and Cr(III), reaction temperature, and reaction time were chosen as independent variables, and their combined effects on the conversion of Cr(III) (response 1) and the yield of chromium methionine (response 2) were investigated. The optimal values of the parameters were found to be the following: ethanol content of aqueous ethanol is 48.5%; n(Met)/n(NaOH)/n(CrCl3 center dot 6H(2)O) = 4.31:4.31:1; reaction time is 127 min; reaction temperature is 81.1 degrees C; initial Cr(III) concentration is 0.25 mol/L. The conversion of Cr(III) and the yield of chromium methionine are up to 99.79% and 99.23%, respectively, under the optimal conditions. The analysis of variance (ANOVA) and regression with R-2 values of 0.9984 for the conversion of Cr(III) and 0.9974 for the yield of chromium methionine illustrate that the experimental results are in good agreement with the predicted values, and the models can be used to predict the synthesis of chromium methionine successfully.