EPIDERMAL GROWTH-FACTOR ENHANCES OOCYTE MATURATION IN PIGS
EPIDERMAL GROWTH-FACTOR ENHANCES OOCYTE MATURATION IN PIGS
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DOI：
10.1002/mrd.1080390106
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发表时间：
1994-09-01
影响因子：
2.5
通讯作者：
FOXCROFT, GR
中科院分区：
文献类型：
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作者：
DING, JC;FOXCROFT, GR
已报道表皮生长因子（EGF）刺激猪卵母细胞的核成熟（Sommer等人，1992年）。本实验的目的是测试EGF单独或与促性腺激素和卵泡壳共培养组合对卵母细胞体外胞质成熟的影响。初步实验测试了EGF在我们的培养体系中刺激卵母细胞核成熟的有效剂量。在0.1、1和10 ng/ml EGF存在下培养的卵母细胞复合体的核成熟率（MII%）分别为67.2 +/- 4.6、81.1 +/- 8.6和80.7 +/- 5.1，显著高于（P < 0.05）在EGF不存在下培养的卵母细胞（18.1 +/- 9.4%）。在主要实验中，使用2 × 2 × 2析因随机完全区组设计来检查EGF（1.0 ng/ml）单独或与促性腺激素和卵泡壳共培养物组合对细胞质成熟的影响。通过精子穿透后卵母细胞使精子核去致密化的能力来评价细胞质成熟。EGF单独作用对卵母细胞胞质成熟无促进作用（P > 0.05）。EGF与促性腺激素对雄性原核形成的促进作用呈显著正交互作用（P < 0.05）。我们的结论是，EGF单独可以刺激猪卵母细胞的核成熟，EGF可以与促性腺激素相互作用，以提高胞质成熟。在体外成熟的猪卵母细胞的细胞质成熟的高水平，可以实现使用EGF，促性腺激素和卵泡壳补充的培养环境的组合。(C)1994 Wiley-Liss，Inc.
Epidermal Growth Factor (EGF) has been reported to stimulate nuclear maturation in porcine oocytes (Sommer et al., 1992). The objective of this experiment was to test the effect of EGF alone or in combination with gonadotropins and follicular shell coculture, on cytoplasmic maturation of oocytes in vitro. A preliminary experiment tested the effective dose of EGF for stimulation of oocyte nuclear maturation in our culture system. Nuclear maturation rates (MII%) of 67.2 +/- 4.6, 81.1 +/- 8.6, and 80.7 +/- 5.1 for oocyte complexes cultured in the presence of 0.1, 1, and 10 ng/ml EGF, respectively, were significantly higher (P < 0.05) than for oocytes cultured in the absence of EGF (18.1 +/- 9.4%). In the main experiment a 2 x 2 x 2 factorial random complete block design was used to examine the effect of EGF (1.0 ng/ml) alone or in combination with gonadotropins and follicular shell coculture on cytoplasmic maturation. Cytoplasmic maturation was evaluated by the ability of oocytes to decondense sperm nuclei after sperm penetration. EGF alone did not stimulate cytoplasmic maturation in oocytes in vitro (P > 0.05). However, EGF showed a positive interaction (P < 0.05) with gonadotropin treatment on male pronuclear formation. We conclude that EGF alone can stimulate nuclear maturation in pig oocytes, and EGF can interact with gonadotropins to enhance cytoplasmic maturation. A high level of cytoplasmic maturation of in vitro matured pig oocytes could be achieved using a combination of EGF, gonadotropin, and follicular shell supplementation to the culture environment. (C) 1994 Wiley-Liss, Inc.