Identification of a unique transcriptional architecture for the sigS operon in Staphylococcus aureus.
Identification of a unique transcriptional architecture for the sigS operon in Staphylococcus aureus.
复制标题
鉴定金黄色葡萄球菌中 sigS 操纵子的独特转录结构。
DOI：
10.1093/femsle/fny108
复制
发表时间：
2018
影响因子：
2.1
通讯作者：
Shaw,LindseyN
中科院分区：
文献类型：
--
作者：
Miller,HalieK;Burda,WhittneyN;Carroll,RonanK;Shaw,LindseyN
Staphylococcus aureuspossess three alternative σ factors, including a lone extracytoplasmic function σ factor, σS. Our group previously identified and characterized this element, mapping threesigSpromoters, demonstrating its inducibility during stress and virulence inducing conditions and demonstrating a role for this factor in disease causation. In the present study, we identify a fourth promoter of thesigSoperon, termed P4, located in a unique position internal to thesigScoding region. Transcriptional profiling revealed that expression from P4 is dominant to the three upstream promoters, particularly upon exposure to chemical stressors that elicit DNA damage and disrupt cell wall stability; each of which have previously been shown to stimulatesigSexpression. Importantly, expression of this fourth promoter, followed by at least one or more of the upstream promoters, is induced during growth in serum and upon phagocytosis by RAW 264.7 murine macrophage-like cells. Finally, we demonstrate that a downstream gene, SACOL1829, bears a large 3΄ UTR that spans thesigS-SACOL1828 coding region, and may serve to compete with the P4 transcript to inhibit σSproduction. Collectively, these findings reveal a unique operon architecture for thesigSlocus that indicates the potential for novel regulatory mechanisms governing its expression.
