Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies.
Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies.
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DOI：
10.1084/jem.155.5.1579
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发表时间：
1982-05-01
期刊：
影响因子：
--
通讯作者：
Wigzell H
中科院分区：
文献类型：
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作者：
Landegren U;Ramstedt U;Axberg I;Ullberg M;Jondal M;Wigzell H
在对人淋巴细胞具有亲和力的七种单克隆抗体中，三种显示出防止细胞毒性T细胞活性，而当在没有补体的情况下应用时，没有一种影响自然杀伤细胞活性。分别对所有T细胞和细胞毒性/抑制性亚群具有亲和力的抗OKT 3和抗Leu-2a均显示通过与效应细胞的相互作用抑制T杀伤。对于抗OKT 3，在游离抗体被洗掉后，抑制仍然存在。相反，抗Leu-2a诱导快速可逆的抑制。使用单细胞测定，抗OKT 3显示降低裂解能力而不影响靶细胞结合，而抗Leu-2a阻止效应物结合靶细胞。
Out of a panel of seven monoclonal antibodies with affinity for human lymphoid cells, three were shown to prevent cytotoxic T cell activity, whereas none affected natural killer cell activity when applied without complement. Anti-OKT3 and anti-Leu-2a, with affinity for all T cells and the cytotoxic/suppressive subset, respectively were both shown to inhibit T killing by their interaction with the effector cell. For anti- OKT3, the inhibition remained after free antibody was washed away. Anti- Leu-2a, in contrast, induced a rapidly reversible inhibition. Using a single cell assay, anti-OKT3 was shown to reduce the lytic ability without affecting target cell binding, whereas anti-Leu-2a prevented the effectors from binding target cells.