Purification and properties of dioldehydrase, and enzyme requiring a cobamide coenzyme.
Purification and properties of dioldehydrase, and enzyme requiring a cobamide coenzyme.
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二醇脱水酶和需要钴酰胺辅酶的酶的纯化和特性。
DOI：
10.1016/s0021-9258(19)67979-2
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
R. Abeles
中科院分区：
文献类型：
--
作者：
H. A. Lee;R. Abeles
丙二醇转化为丙醛和乙二醇转化为乙醛已显示在产气荚膜梭菌（ATCC 8724）的粗提取物中发生（I），并且已证明需要钴酰胺辅酶。随后，报道了几个同样需要钴胺辅酶的类似反应。乳酸杆菌（2）的提取物将甘油转化为1%羟基丙醛。研究了A.产气菌（572，P2 H）也有描述（3）。我们现在已经从A.产气菌（ATCC 8724）200倍。本文介绍了该酶的纯化方法及部分性质。该酶将被称为二油酸甘油酯酶。
The conversion of propanediol to propionaldehyde and ethylene glycol to acetaldehyde has been shown to occur in crude extracts of A erobacter aerogenes (ATCC 8724)(I), and the requirement of a cobamide coenzyme has been demonstrated. Subsequently, several similar reactions which also require a cobamide coenzyme were reported. Extracts of Lactobacillus (2) carry out the conversion of glycerol to/% hydrosypropionaldehyde. The conversion of ethylene glycol to aldol in extracts of A. aerogenes (572, P2H) also has been described (3). We have now purified the enzyme from A. aerogenes(ATCC 8724) 200-fold. The method of purification as well as some properties of the purified enzyme will be described in this paper. The enzyme will be referred to as dioldehydrase.