Reduced translocation in 2,4-D-resistant oriental mustard populations (Sisymbrium orientale L.) from Australia
Reduced translocation in 2,4-D-resistant oriental mustard populations (Sisymbrium orientale L.) from Australia
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DOI：
10.1002/ps.4845
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发表时间：
2018-06-01
影响因子：
4.1
通讯作者：
Preston, Christopher
中科院分区：
文献类型：
--
作者：
Hue Thi Dang;Malone, Jenna M.;Preston, Christopher
从南澳布劳顿港采集的两个东方芥菜群体（P2和P13）被鉴定为抗2，4-D。研究了这些群体对2，4-D的抗性水平、抗性机理和抗性遗传方式，结果表明，P2和P13群体在田间水平（600 g a.e.）ha（-1））。在50%死亡率（LD 50）所需的剂量下，P2和P13的抗性分别是易感人群（S1和S2）的81倍和67倍。在潜在靶位点基因（ABP、TIR 1和AFB 5）序列中未检测到预测的氨基酸修饰。与敏感群体相比，抗性群体的2，4-D转运减少，处理后72 h，77%的[C-14] 2，4-D保留在处理的叶片中，而处理后72 h为32%。对2，4-D的抗性编码在核基因组上，并且是显性的，因为所有F-2个体对2，4-D的反应与抗性生物型相似。F-2表型的分离符合3：1（R：S）的遗传模型。结论东方芥菜对2，4-D的抗性可能是由于处理叶片中2，4-D的转运减少所致。2，4-D抗性的遗传是由一个具有高显性水平的单基因赋予的。(c)2017年化学工业协会
BACKGROUNDTwo oriental mustard populations (P2 and P13) collected from Port Broughton, South Australia were identified as resistant to 2,4-D. The level of resistance, mechanism and the mode of inheritance for 2,4-D resistance in these populations were investigated.RESULTSPopulations P2 and P13 were confirmed to be resistant to 2,4-D at the field rate (600g a.e. ha(-1)). P2 and P13 were 81- and 67-fold more resistant than the susceptible populations (S1 and S2) at the dose required for 50% mortality (LD50), respectively. No predicted amino acid modification was detected in sequences of potential target-site genes (ABP, TIR1 and AFB5). Resistant populations had reduced 2,4-D translocation compared with the susceptible populations, with 77% of [C-14]2,4-D retained in the treated leaf versus 32% at 72h after treatment. Resistance to 2,4-D is encoded on the nuclear genome and is dominant, as the response to 2,4-D of all F-2 individuals were similar to the resistant biotypes. The segregation of F-2 phenotypes fitted a 3: 1 (R: S) inheritance model.CONCLUSIONResistance to 2,4-D in oriental mustard is likely due to reduced translocation of 2,4-D out of the treated leaf. Inheritance of 2,4-D resistance is conferred by a single gene with a high level of dominance. (c) 2017 Society of Chemical Industry