Sulforaphane attenuates di-N-butylphthalate-induced reproductive damage in pubertal mice: Involvement of the Nrf2-antioxidant system
Sulforaphane attenuates di-N-butylphthalate-induced reproductive damage in pubertal mice: Involvement of the Nrf2-antioxidant system
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萝卜硫素可减轻青春期小鼠邻苯二甲酸二正丁酯引起的生殖损伤：Nrf2 抗氧化系统的参与
DOI：
10.1002/tox.22413
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发表时间：
2017-07-01
影响因子：
4.5
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Jiang, Xu-ping;Tang, Jing-yuan;Zhang, Wei
邻苯二甲酸二正丁酯（DBP）是用于塑料涂料和化妆品工业的普遍存在的环境污染物。它对身体健康，特别是男性生殖系统有毒性作用。本文研究了DBP对青春期小鼠雄性生殖系统的影响，并探讨了莱菔硫烷（SFN）的保护作用。结果表明，DBP显著降低了肛门生殖器距离、睾丸重量、精子计数和活动率、血浆和睾丸睾酮水平，并显著增加了氧化应激、精子畸形和睾丸细胞凋亡。SFN补充改善了这些影响。DBP刺激后，转录因子核因子红细胞相关因子2（Nrf 2）与其靶基因，如HO-1和NQO 1一起适应性增加。SFN上调Nrf 2可降低DBP介导的细胞内氧化毒性，并增加睾酮分泌和精子发生，而DBP可降低睾酮分泌和精子发生。这些发现表明SFN可以通过Nrf 2相关途径减轻DBP诱导的青春期小鼠生殖损伤。
di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis. SFN supplementation ameliorated these effects. After DBP stimulation, the transcription factor nuclear factor erythroid-related factor 2 (Nrf2) was adaptively increased together with its target genes, such as HO-1 and NQO1. Upregulation of Nrf2 by SFN reduced the DBP-mediated intracellular oxidative toxicity and also increased testosterone secretion and spermatogenesis, which were decreased by DBP. These findings indicate that SFN can attenuate DBP-induced reproductive damage in pubertal mice via Nrf2-associated pathways.