STRUCTURAL INFORMATION ON HYALURONIC-ACID SOLUTIONS AS STUDIED BY PROBE DIFFUSION EXPERIMENTS
STRUCTURAL INFORMATION ON HYALURONIC-ACID SOLUTIONS AS STUDIED BY PROBE DIFFUSION EXPERIMENTS
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DOI：
10.1021/ma00079a021
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发表时间：
1994-01-03
期刊：
影响因子：
5.5
通讯作者：
DU, M
中科院分区：
文献类型：
--
作者：
DE SMEDT, SC;LAUWERS, A;DU, M
采用光漂白后荧光恢复技术（FRAP）测定异硫氰酸荧光素（FITC）-葡聚糖在稀释、半稀释和浓缩透明质酸溶液中的扩散系数（D）。扩散系数随透明质酸浓度的增加而减小，符合普适定标方程D/D-0 = exp（-ac（v））。进行扩散实验，以获得透明质酸溶液中的瞬态网络结构的结构信息。根据标度律，确定平均筛孔尺寸Xi的浓度依赖性为Xi，类似于c（-0.58+/-0.07）。此外，估计了平均xi值。将Xi的浓度依赖性和Xi的绝对值与从对透明质酸溶液进行的流变学实验获得的结构信息进行比较。即使准确的结果被发现的浓度依赖性的Xi，有一个半定量之间的关系，估计从扩散实验和流变实验的Xi值。
The fluorescence recovery after photobleaching technique (FRAP) was used to measure the diffusion coefficients (D) of fluorescein isothiocyanate (FITC)-dextrans in diluted, semidiluted, and concentrated hyaluronic acid solutions. The decrease of the diffusion coefficients as the hyaluronic acid concentration increases was consistent with the universal scaling equation D/D-0 = exp(-ac(v)). The diffusion experiments were carried out to obtain structural information on the transient network structure in hyaluronic acid solutions. On the basis of scaling laws, the concentration dependence of the average mesh size xi was determined as xi similar to c(-0.58+/-0.07). Additionally, the average xi-values were estimated. The concentration dependence of xi and the absolute vlaues for xi were compared with structural information obtained from theological experiments performed on hyaluronic acid solutions. Even though accurate results were found for the concentration dependence of xi, there was a semiquantitative relation between the xi-values estimated from the diffusion experiments and the theological experiments.