Comment to Shinohara et al. (1991) FEBS Letters 293, 1731̄74 The uncoupling protein is not expressed in rat liver

Comment to Shinohara et al. (1991) FEBS Letters 293, 1731̄74 The uncoupling protein is not expressed in rat liver
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Shinohara 等人的评论 (1991) FEBS Letters 293, 1731̄74 解偶联蛋白在大鼠肝脏中不表达

DOI:
10.1016/0014-5793(92)80496-4
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发表时间:
1992
期刊:
影响因子:
3.5
通讯作者:
F. Bouillaud
F. Bouillaud
中科院分区:
生物学3区
文献类型:
--
作者:
D. Ricquier;S. Raimbault;O. Champigny;B. Miroux;F. Bouillaud

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使用北方印迹分析、纯化抗体免疫印迹和聚合酶链反应分析,我们无法在对照、冷暴露或新生大鼠的肝脏中检测解偶联蛋白-UCP或其mRNA。证实了该蛋白在棕色脂肪细胞中的独特表达。这些数据反驳了最近关于UCP在大鼠肝脏中表达的令人惊讶的报道(Shinohara(1991)FEBS Lett. 293,173-174)。此外,我们报告说,这些作者获得的杂交信号可能是非特异性的,这是由于UCP cDNA探针的3′非编码结构域。
Using Northern blot analysis, immunoblotting with purified antibodies and Polymerase Chain Reaction analysis, we were unable to detect the Uncoupling Protein‐UCP or its mRNA in liver of control, cold‐exposed or newborn rats. The unique expression of this protein in brown adipocytes was confirmed. These data refute the surprising recent report on UCP expression in rat liver (Shinohara (1991) FEBS Lett. 293, 173–174). Moreover we report that the hybridization signal obtained by these authors is probably non‐specific and due to the 3′ non‐coding domain of the UCP cDNA probe.
大鼠18S核糖体核糖核酸基因的核苷酸序列和18S核糖体核糖核酸二级结构的建议。
DOI: --
发表时间: 1984
期刊: The Journal of biological chemistry
影响因子: --
作者:
Chan,YL;Gutell,R;Noller,HF;Wool,IG
通讯作者: Wool,IG