DIFFERENTIATION OF ODONTOBLASTS IN GRAFTED RECOMBINANTS OF MURINE EPITHELIAL ROOT SHEATH AND DENTAL MESENCHYME
DIFFERENTIATION OF ODONTOBLASTS IN GRAFTED RECOMBINANTS OF MURINE EPITHELIAL ROOT SHEATH AND DENTAL MESENCHYME
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DOI：
10.1016/0003-9969(89)90043-5
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发表时间：
1989-01-01
影响因子：
3
通讯作者：
KOLLAR, EJ
中科院分区：
文献类型：
--
作者：
THOMAS, HF;KOLLAR, EJ
用胰蛋白酶从出生后5天的CD-1小鼠的第一磨牙分离上皮根鞘（ERS）。分离后，ERS细胞在体外保持活力，培养物的免疫组化检查证实了上皮表型和间充质污染的情况下。离体ERS和牙乳头的降解剂导致牙乳头细胞内的成牙本质细胞分化，并在眼内移植2周后形成根状牙本质碎片。这些结果表明ERS对牙乳头细胞有诱导作用。
The epitheial root sheath (ERS) was isolated from first molars of 5-day-old post-natal CD-1 mice using trypsin. After isolation, ERS cells remained viable in vitro and immunohistochemical examination of cultures confirmed the epithelial phenotype and the absence of mesenchymal contamination. Recombinants of isolated ERS and dental papilla resulted in odontoblast differentiation within cells of the dental papilla, and the formation of root-like fragments of dentine after 2 weeks of intra-ocular grafting. These findings indicate the inductive influence of the ERS on dental papilla cells.