Integrin evolution: Insights from ascidian and teleost fish genomes
Integrin evolution: Insights from ascidian and teleost fish genomes
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DOI：
10.1016/j.matbio.2005.01.003
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发表时间：
2005-04-01
期刊：
影响因子：
6.9
通讯作者：
Johnson, MS
中科院分区：
文献类型：
--
作者：
Huhtala, M;Heino, J;Johnson, MS
整合素是所有后生动物细胞粘附所必需的α - β异二聚体受体家族。在人类中，这个家族由18个α亚基和8个β亚基组成，它们结合起来形成24个二聚体。在此，我们基于24种无脊椎动物和脊椎动物的序列，对α和β整合素亚基进行了系统发育重建，包括尾脊索动物海鞘和硬骨鱼的全基因组测序。两种基因组都含有插入α 1结构域的整合素亚基。对于先前在海鞘（Halocynthia roretzi）中发现的一个含有整合素α亚基的α I结构域，Ciona α I结构域缺少哺乳动物胶原受体的独特特征，并且在系统发育树中与所有脊椎动物α I结构域整合素分离，形成了一个具有α I结构域的α亚基新亚群。每个河豚α 1结构域序列都具有胶原受体α 1结构域的特征，但在河豚中没有发现白细胞特异性α 1结构域。比较蛋白质模型表明，这些鱼α 1结构域中的一些在结构上与胶原蛋白三螺旋中的GFOGER序列结合是兼容的。(c) 2005 Elsevier B.V./国际基质生物学学会。版权所有。
Integrins are a family of alpha beta heterodimeric receptors essential to cell adhesion in all metazoans. In humans, the family consists of 18 alpha and 8 beta subunits that combine to form 24 dimers. Here, we present phylogenetic reconstructions for the alpha and beta integrin subunits based on sequences from 24 invertebrate and vertebrate species, including the fully sequenced genomes of the ascidian Ciona intestinalis (a urochordate) and the pufferfish Takifugu rubripes (a teleost). Both genomes contain integrin alpha subunits that have the inserted alpha I domain. As for the one alpha I domain containing integrin alpha subunit discovered earlier from the ascidian Halocynthia roretzi, the Ciona alpha I domains are missing the distinctive characteristics of mammalian collagen receptors and segregate from all vertebrate alpha I domain integrins in a phylogenetic tree, forming a new subgroup of alpha subunits with alpha I domains. Each of the pufferfish alpha I domain sequences does have characteristics of the collagen receptor alpha I domains, but no leukocyte-specific alpha I domains were found in pufferfish. Comparative protein modeling suggests that several of these fish alpha I domains are structurally compatible with binding to a GFOGER sequence in a collagen triple helix. (c) 2005 Elsevier B.V./International Society of Matrix Biology. All rights reserved.