Amphiphilic peptide coated superparamagnetic iron oxide nanoparticles for in vivo MR tumor imaging
Amphiphilic peptide coated superparamagnetic iron oxide nanoparticles for in vivo MR tumor imaging
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DOI：
10.1039/c6ra07380h
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发表时间：
2016-05
期刊：
影响因子：
3.9
通讯作者：
A. Ozdemir;Melis Sardan Ekiz;A. Dilli;M. O. Guler;A. Tekinay
中科院分区：
文献类型：
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作者：
A. Ozdemir;Melis Sardan Ekiz;A. Dilli;M. O. Guler;A. Tekinay
磁共振成像（MRI）是一种非侵入性成像技术，可提供高空间分辨率和深度，并具有明显的软组织对比度，用于体内成像。各种各样的策略已被用来提高MRI的诊断价值，并在早期发现组织异常。超顺磁性氧化铁纳米颗粒（SPION）由于其体积小、功能多样和生物相容性好而被认为是有效成像的合适候选物。在这里，我们证明了涂层SPION与脯氨酸丰富的两亲性肽分子通过非共价相互作用导致水分散的混合系统适合作为MRI造影剂。用人血管内皮细胞（HUVEC）和雌激素受体（ER）阳性的人乳腺癌（MCF-7）细胞评价两亲性肽包被的SPION（SPION/K-PA）的细胞活力和摄取。在患有乳腺肿瘤的Sprague-Dawley大鼠中分析SPION/K-PA作为MRI造影剂的效率。MR成像显示SPION/K-PA在肿瘤组织中有效蓄积，增强了其成像潜力。尽管在网状内皮系统器官（RES）中观察到纳米颗粒，特别是在给药后立即在肝脏和肾脏中观察到纳米颗粒，但这些器官中的MR信号强度在1小时后减弱，纳米颗粒随后在两周内从这些器官中清除。组织学观察还验证了纳米颗粒在4小时时在肿瘤组织中的积累以及两周后从健康和荷瘤大鼠的器官中的生物消除。
Magnetic resonance imaging (MRI) is a noninvasive imaging technique that provides high spatial resolution and depth with pronounced soft-tissue contrast for in vivo imaging. A broad variety of strategies have been employed to enhance the diagnostic value of MRI and detect tissue abnormalities at an earlier stage. Superparamagnetic iron oxide nanoparticles (SPIONs) are considered to be suitable candidates for effective imaging due to their small size, versatile functionality and better biocompatibility. Here, we demonstrate that coating SPIONs with proline-rich amphiphilic peptide molecules through noncovalent interactions leads to a water-dispersed hybrid system suitable as an MRI contrast agent. Cellular viability and uptake of amphiphilic peptide coated SPIONs (SPION/K-PA) were evaluated with human vascular endothelial cells (HUVEC) and estrogen receptor (ER) positive human breast adenocarcinoma (MCF-7) cells. The efficiency of SPION/K-PA as MRI contrast agents was analyzed in Sprague-Dawley rats with mammary gland tumors. MR imaging showed that SPION/K-PA effectively accumulated in tumor tissues, enhancing their imaging potential. Although nanoparticles were observed in reticuloendothelial system organs (RES) and especially in the liver and kidney immediately after administration, the MR signal intensity in these organs diminished after 1 h and nanoparticles were subsequently cleared from these organs within two weeks. Histological observations also validated the accumulation of nanoparticles in tumor tissue at 4 h and their bioelimination from the organs of both healthy and tumor-bearing rats after two weeks.