Ureaplasma-eukaryotic cell interactions in vitro.
Ureaplasma-eukaryotic cell interactions in vitro.
复制标题
体外解脲支原体-真核细胞相互作用。
DOI：
10.1097/00006454-198611010-00026
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Kotani,H
中科院分区：
文献类型：
--
作者：
McGarrity,GJ;Kotani,H
: Ureaplasmas from six animal species were inoculated into cell cultures derived from human, simian and murine hosts. All ureaplasmas infected at least two of the cell cultures tested. Supplemental medium urea was not a requirement for cell culture infection, and Ureaplasma growth in cell cultures occurred in as little urea as 4 [mu] g/ml, similar to findings in broth-propagated organisms. These findings are of potential significance due to an observation (HM Jernigan, Jr: Exp Eye Res 37: 551, 1983) that certain tissues produce urea locally. This may explain why ureaplasmas can be isolated from tissues outside the urinary tract where large amounts of urea are not routinely encountered. Ureaplasmas generally increased from titers of approximately 102 to 104 to ones of 105 to 106, but occasionally 107, color changing-units/ml within 2 days. Apparently a cell culture product is required for Ureaplasma growth. In several systems, Ureaplasma infection persisted and Ureaplasma-infected cell cultures could be passaged.
