Whole-blood sorting, enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming
Whole-blood sorting, enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming
复制标题
全血分选，富集和原位免疫标记细胞亚群使用声学微流
DOI：
10.1038/micronano.2017.85
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发表时间：
2018-02-26
影响因子：
7.9
通讯作者：
Lee, Abraham P.
中科院分区：
文献类型：
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作者：
Garg, Neha;Westerhof, Trisha M.;Lee, Abraham P.
由于造血细胞群、核酸、代谢物和蛋白质的复杂组成，分析未稀释的人类全血是一项挑战。我们提出了一种新型多功能微流体声流平台，能够对全血中的细胞亚群进行分类、富集和原位识别。这个基于侧腔声换能器 (LCAT) 的单一设备平台能够 (1) 将未稀释的供体全血分选为其细胞亚群（血小板、红细胞和白细胞），(2) 以稀有细胞相关浓度 (10 mL(-1)) 富集和检索供体全血中掺入的乳腺癌细胞 (MCF-7)，以及 (3) 芯片上免疫荧光标记，用于通过已知的标记物表达模式检测特定目标细胞群。因此，我们的方法展示了一个紧凑的系统，该系统将上游样品处理与下游分离/富集集成在一起，以进行基于血液的诊断和液体活检血液采样的多参数细胞分析。
Analyzing undiluted whole human blood is a challenge due to its complex composition of hematopoietic cellular populations, nucleic acids, metabolites, and proteins. We present a novel multi-functional microfluidic acoustic streaming platform that enables sorting, enrichment and in situ identification of cellular subsets from whole blood. This single device platform, based on lateral cavity acoustic transducers (LCAT), enables (1) the sorting of undiluted donor whole blood into its cellular subsets (platelets, RBCs, and WBCs), (2) the enrichment and retrieval of breast cancer cells (MCF-7) spiked in donor whole blood at rare cell relevant concentrations (10 mL(-1)), and (3) on-chip immunofluorescent labeling for the detection of specific target cellular populations by their known marker expression patterns. Our approach thus demonstrates a compact system that integrates upstream sample processing with downstream separation/enrichment, to carry out multi-parametric cell analysis for blood-based diagnosis and liquid biopsy blood sampling.