Validity of Tissue Microarray by Immunohistochemistry
Validity of Tissue Microarray by Immunohistochemistry
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DOI：
10.7754/clin.lab.2014.141102
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发表时间：
2015-01-01
影响因子：
0.7
通讯作者：
Ozono, Seiichiro
中科院分区：
文献类型：
--
作者：
Fujita, Hiromi;Takayama, Tatsuya;Ozono, Seiichiro
背景资料：组织微阵列（TMAs）提取指定区域的组织石蜡块在几个单位，直径为毫米的圆柱形的方式，阵列几十个这些组织标本，然后重新包埋它们。在这里，一个TMA被用来分析肾细胞癌（RCC）标本与抗FABP 7和抗Brn 2 antibodies.Methods：石蜡包埋标本从114 RCC患者进行了免疫染色与抗FABP 7和抗Brn 2抗体检查TMA移植物的染色之间的协议率相比，传统的组织切片移植。结果：FABP 7抗体阳性率为74%，Brn 2抗体阳性率为57%。无论肿瘤大小，每种抗体的一致率为100% extraction.Conclusions：TMA切片的免疫染色可能是有效的RCC标本的分析。
Background: Tissue microarrays (TMAs) extract designated areas of tissue paraffin blocks in several units that are millimeters in diameter in a cylindrical fashion, array dozens of these tissue specimens, and then re-embed them. Here, a TMA was utilized to analyze renal cell carcinoma (RCC) specimens with anti-FABP7 and anti-Brn2 antibodies.Methods: Paraffin-embedded specimens from 114 RCC patients were immunostained with anti-FABP7 and anti-Brn2 antibodies to examine the rate of agreement between the staining of TMA grafts compared to conventional tissue slice grafts. The staining area of the tumor was also examined.Results: The positive ratio of anti-FABP7 was 74% and of anti-Brn2 was 57%. The rate of agreement of each antibody was 100% regardless of tumor size before extraction.Conclusions: Immunostaining of TMA slices might be effective for the analysis of RCC specimens.