YEAST MATING PHEROMONE ALPHA-FACTOR INHIBITS ADENYLATE-CYCLASE
YEAST MATING PHEROMONE ALPHA-FACTOR INHIBITS ADENYLATE-CYCLASE
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DOI：
10.1073/pnas.77.4.1898
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
THORNER, J
中科院分区：
文献类型：
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作者：
LIAO, H;THORNER, J
信息素。由酿酒葡萄球菌的细胞分泌的。交配型，在G1时同步相反交配型（a细胞），作为两种细胞融合的前奏。在体外，。因子以剂量依赖的方式抑制这些细胞的膜结合腺苷酸环化酶。此外，有一类细胞（ste5）在23度的温度下正常生长。或34.度。C，但不能对。alpha做出反应。在较高温度下交配的因子具有不受α抑制的腺苷酸环化酶活性。因子34度。C，但对23度的抑制完全敏感。C.此外，在细胞培养基中加入环状AMP可缩短信息素诱导的G1阻滞期。α对腺苷酸环化酶活性的抑制作用。因子可以至少部分地构成信息素在体内作用的生化模式。
The pheromone .alpha. factor, secreted by S. cerevisiae cells of the .alpha. mating type, serves to synchronize the opposite mating type (a cells) at G1 as a prelude to fusion of the 2 cell types. In vitro, .alpha. factor inhibited the membrane-bound adenylate cyclase of these cells in a dose-dependent manner. Moreover, one class (ste5) of a cell mutants that grow normally at either 23.degree. or 34.degree. C but that are unable to respond to .alpha. factor or to mate at the higher temperature possessed an adenylate cyclase activity that was not inhibited by .alpha. factor at 34.degree. C but was fully sensitive to inhibition at 23.degree. C. Furthermore, addition of cyclic AMP to a cell culture medium shortened the period of pheromone-induced G1 arrest. Inhibition of adenylate cyclase activity by .alpha. factor may constitute, at least in part, the biochemical mode of action of the pheromone in vivo.