Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana
Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana
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DOI：
10.1080/19491034.2017.1279774
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发表时间：
2017-03
期刊：
影响因子：
3.7
通讯作者：
K. Tamura;Y. Fukao;N. Hatsugai;F. Katagiri;I. Hara-Nishimura
中科院分区：
文献类型：
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作者：
K. Tamura;Y. Fukao;N. Hatsugai;F. Katagiri;I. Hara-Nishimura
摘要 核孔复合物 (NPC) 包含 30 多种核孔蛋白 (Nups)。 NPC 介导核质和细胞质之间的大分子运输，但在高等植物中，个体 Nups 的具体作用知之甚少。在这里，我们证明了被子植物特有的新型核孔蛋白（称为 Nup82）与 Nup136（NPC 核篮的组成部分）以冗余方式在水杨酸依赖性防御中发挥作用。拟南芥 Nup82 与 Nup136 的 N 端一半具有相似的氨基酸序列，并且 Nup82-GFP 融合体位于核膜上。免疫沉淀和双分子荧光互补分析表明，Nup82 与 NPC 成分 Nup136 和 RAE1 相互作用。双敲除突变体nup82 nup136表现出严重的生长缺陷，而单敲除突变体nup82则没有，这表明Nup82与Nup136功能冗余。 nup82 nup136 损害苯并噻二唑（水杨酸类似物）诱导的对有毒细菌丁香假单胞菌的抵抗力。番茄DC3000。此外，nup82 nup136 的转录组分析表明，Nup82 和 Nup136 的缺陷会导致免疫相关基因的显着下调。这些结果表明，Nup82 和 Nup136 通过信号分子的核转运过多地参与水杨酸响应基因的转录调节。
ABSTRACT The nuclear pore complex (NPC) comprises more than 30 nucleoporins (Nups). NPC mediates macromolecular trafficking between the nucleoplasm and the cytoplasm, but specific roles of individual Nups are poorly understood in higher plants. Here, we show that the novel nucleoporin unique to angiosperm plants (designated as Nup82) functions in a salicylic acid-dependent defense in a redundant manner with Nup136, which is a component of the nuclear basket in the NPC. Arabidopsis thaliana Nup82 had a similar amino acid sequence to the N-terminal half of Nup136 and a Nup82-GFP fusion was localized on the nuclear envelope. Immunoprecipitation and bimolecular fluorescence complementation analyses revealed that Nup82 interacts with the NPC components Nup136 and RAE1. The double knockout mutant nup82 nup136 showed severe growth defects, while the single knockout mutant nup82 did not, suggesting that Nup82 functions redundantly with Nup136. nup82 nup136 impaired benzothiadiazole (an analog of salicylic acid)-induced resistance to the virulent bacteria Pseudomonas syringae pv. tomato DC3000. Furthermore, transcriptome analysis of nup82 nup136 indicates that deficiency of Nup82 and Nup136 causes noticeable downregulation of immune-related genes. These results suggest that Nup82 and Nup136 are redundantly involved in transcriptional regulation of salicylic acid-responsive genes through nuclear transport of signaling molecules.