Expression Parameters of the Polycomb Group Proteins BMI1, SUZ12, RING1 and CBX7 in Urothelial Carcinoma of the Bladder and Their Prognostic Relevance
Expression Parameters of the Polycomb Group Proteins BMI1, SUZ12, RING1 and CBX7 in Urothelial Carcinoma of the Bladder and Their Prognostic Relevance
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DOI：
10.1159/000170879
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Weikert, S.
中科院分区：
文献类型：
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作者：
Hinz, S.;Kempkensteffen, C.;Weikert, S.
背景资料：Polycomb组（PCG）蛋白是参与控制细胞增殖和肿瘤发生的表观遗传转录抑制因子。本研究旨在检查PCG家族成员BMI 1、SUZ 12、RING 1和CBX 7的mRNA肿瘤水平是否与膀胱尿路上皮癌的组织病理学参数相关，以及它们是否可以提供肿瘤切除后的预后信息。研究方法：采用实时荧光定量RT-PCR方法检测93例膀胱尿路上皮癌患者手术切除肿瘤组织中BMI 1、SUZ 12、RING 1和CBX 7的相关基因表达。表达数据与病理变量和结果相关。结果：PCG家族成员BMI 1、SUZ 12、RING 1和CBX 7在膀胱尿路上皮癌中表达普遍。相对CBX 7 mRNA表达水平从浅表（>= pTa）到浸润性（pT 1），最后到肌肉浸润性（>= pT 2）肿瘤逐渐降低（p = 0.008）。此外，CBX 7表达与肿瘤分级在统计学上显著相关（p = 0.04）。研究的其他PCG组分的mRNA水平与组织病理学肿瘤特征或肿瘤复发无关。结论：CBX 7的表达水平与膀胱尿路上皮癌的肿瘤分期和分级的进展呈负相关，表明CBX 7的下调指示侵袭性尿路上皮癌表型。版权所有（C）2008 S. Karger AG，巴塞尔
Background: The Polycomb group (PCG) proteins are epigenetic transcriptional repressors involved in the control of cellular proliferation and oncogenesis. This study aimed at examining whether mRNA tumor levels of the PCG family members BMI1, SUZ12, RING1, and CBX7 relate to histopathological parameters in urothelial carcinomas of the bladder and whether they may provide prognostic information following tumor resection. Methods: The relative gene expression of BMI1, SUZ12, RING1, and CBX7 was analyzed by real-time RT-PCR in tumor tissue obtained from 93 patients with urothelial carcinoma of the bladder undergoing surgical treatment. Expression data was correlated with pathological variables and outcome. Results: PCG family members BMI1, SUZ12, RING1, and CBX7 are commonly expressed in urothelial carcinomas of the bladder. The relative CBX7 mRNA expression levels gradually decreased from superficial (>= pTa) to invasive (pT1) and finally to muscle-invasive (>= pT2) tumors (p = 0.008). Furthermore, CBX7 expression was statistically significantly correlated with tumor grade (p = 0.04). No correlation of mRNA levels with histopathological tumor features or tumor recurrence was observed for the other PCG components investigated. Conclusion: Expression levels of CBX7 inversely correlate with the progression of tumor stage and grade in urothelial carcinomas of the bladder, suggesting that downregulation of CBX7 indicates aggressive urothelial carcinoma phenotype. Copyright (C) 2008 S. Karger AG, Basel