IDENTIFICATION AND QUANTIFICATION OF HIGHLY MUTAGENIC NITROACETOXYPYRENES AND NITROHYDROXYPYRENES IN DIESEL-EXHAUST PARTICLES
IDENTIFICATION AND QUANTIFICATION OF HIGHLY MUTAGENIC NITROACETOXYPYRENES AND NITROHYDROXYPYRENES IN DIESEL-EXHAUST PARTICLES
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DOI：
10.1016/0165-1218(85)90092-8
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
OHNISHI, Y
中科院分区：
文献类型：
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作者：
MANABE, Y;KINOUCHI, T;OHNISHI, Y
收集、提取和分馏重型疾病废气颗粒物，将其分成乙醚可溶的中性、酸性和碱性馏分。用鼠伤寒沙门氏菌菌株TA 100、TA 98、TA 98 NR和TA 98/1，8-DNP 6在存在和不存在9000 ×g来自Aroclor诱导的大鼠肝脏的线粒体后上清液（S9混合物）。在不存在S9混合物的情况下，中性和酸性组分显示出TA 98的高致突变性，酸性组分具有最高的比活性。在不存在S9混合物的情况下，TA 98中粗品、中性和酸性组分的致突变性大于TA 98 NR和TA 98/1，8-DNP 6。化学合成的硝基乙酰氧基芘和硝基羟基芘分馏成中性和酸性馏分，分别。用高效液相色谱法对这些硝基芳烃进行了纯化，并用4株菌株测定了它们的致突变性。在不存在S9混合物的情况下，使用TA 98，1-硝基-3-乙酰氧基芘、1-硝基-6/8-乙酰氧基芘、1-硝基-3-羟基芘、1-硝基-6/8-羟基芘分别诱导16700、336、992、94个His+回复突变体/平板/nmole。在不存在S9混合物的情况下，这些硝基芳烃的致突变性水平在TA 98中最高，在TA 98/1，8-DNP 6中最低，在TA 98 NR中居中。采用气相色谱-质谱联用技术和气相色谱-质谱联用技术对柴油机排气颗粒物中的中性组分和酸性组分进行了分析。中性组分中含有硝基乙酰氧基芘、1-硝基芘和1，6-二硝基芘，酸性组分中含有硝基羟基芘。在不存在S9混合物的情况下，1-硝基-3-乙酰氧基芘、1-硝基芘、1，6-二硝基芘和1-硝基-3-羟基芘的含量分别为6.3、62、0.81和70 ng/mg粗提物，分别占TA 98粗提物致突变性的12%、3.6%、8.0%和9.0%。
Heavy-duty diseal-exhaust particles were collected, extracted and fractionated into diethyl ether-soluble neutral, acidic and basic fractions. The mutagenicity of these fractions was measured with Salmonella typhimurium strains TA100, TA98, TA98NR and TA98/1,8-DNP6 in the presence and absence of a 9000 .times. g post-mitochondrial supernatant from Aroclor-induced rat liver (S9 mix). The neutral and acidic fractions showed high mutagenicity with TA98 in the absence of S9 mix, the acidic fraction having the highest specific activity. In the absence of S9 mix, the mutagenicity of crude, neutral and acidic fractions was greater in TA98 than in TA98NR and TA98/1,8-DNP6. Chemically-synthesized nitroacetoxypyrenes and nitrohydroxypyrenes were fractionated into the neutral and acidic fractions, respectively. These nitroarenes were purified by high-performance liquid chromatography and their mutagenicity was measured with the 4 strains. With TA98 in the absence of S9 mix, 1-nitro-3-acetoxypyrene, 1-nitro-6/8-acetoxypyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6/8-hydroxypyrene induced 16700, 336, 992, 94 His+ revertants per plate per nmole, respectively. In the absence of S9 mix, the level of mutagenicity of these nitroarenes was highest in TA98, lowest in TA98/1,8-DNP6 and intermediate in TA98NR. The neutral and acidic fractions of diesel-exhaust particles were analyzed by gas chromatography-mass spectrometry and gas chromatography-mass fragmentography. The neutral fraction was found to contain nitroacetoxypyrenes, 1-nitropyrene, 1,6-dinitropyrene, while nitrohydroxypyrenes were detected in the acidic fraction. The amounts of 1-nitro-3-acetoxypyrene, 1-nitropyrene, 1,6-dinitropyrene and 1-nitro-3-hydroxyprene were 6.3, 62, 0.81, and 70 ng per mg of crude extract, and accounted for 12, 3.6, 8.0, and 9.0% respectively, of mutagenicity of the crude extract in TA98 in the absence of S9 mix.