Effects of Aldehydes on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans
Effects of Aldehydes on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans
复制标题
醛对产油酵母发酵毛孢子菌生长和脂质积累的影响
DOI：
10.1021/jf104320b
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发表时间：
2011-05-11
影响因子：
6.1
通讯作者：
Zong, Min-hua
中科院分区：
文献类型：
--
作者：
Huang, Chao;Wu, Hong;Zong, Min-hua
首次研究了木质纤维素水解液中具有代表性的5种醛对产油酵母发酵毛孢子菌生长和脂肪积累的影响。疏水性与醛的毒性无关，5-羟甲基呋喃甲醛的毒性比芳香醛和呋喃甲醛小。芳香醛的二元组合产生协同抑制作用，而呋喃和芳香醛的组合反而减弱了抑制作用。由于醛的存在和糖耗速率的降低，发酵曲霉利用木糖的滞后期较长，但在醛的存在下，尤其是在低浓度时，发酵木糖的利用率更高。苹果酸酶活性的变化与脂肪积累的延缓无关。此外，醛对细胞生长的抑制作用更多地依赖于接种量、温度和初始pH，而不是脂肪含量。
The effects of five representative aldehydes in lignocellulosic hydrolysates on the growth and the lipid accumulation of oleaginous yeast Trichosporon fermentans were investigated for the first time. There was no relationship between the hydrophobicity and the toxicity of aldehyde, and 5-hydroxymethylfurfural was less toxic than aromatic aldehydes and furfural. Binary combination of aromatic aldehydes caused a synergistic inhibitory effect, but combination of furan and aromatic aldehydes reduced the inhibition instead. A longer lag phase was found due to the presence of aldehydes and the decrease of sugar consumption rate, but more xylose was utilized by T. fermentans in the presence of aldehydes, especially at their low concentrations. The variation of malic enzyme activity was not related to the delay of lipid accumulation. Furthermore, the inhibition of aldehydes on cell growth was more dependent on inoculum size, temperature, and initial pH than that on lipid content.