Erratum: Author Correction: Age-associated changes in the impact of sex steroids on influenza vaccine responses in males and females.
Erratum: Author Correction: Age-associated changes in the impact of sex steroids on influenza vaccine responses in males and females.
复制标题
勘误：作者更正：性类固醇对男性和女性流感疫苗反应影响的年龄相关变化。
DOI：
10.1038/s41541-019-0130-8
复制
发表时间：
2019
期刊：
影响因子：
9.2
通讯作者：
Klein,SabraL
中科院分区：
文献类型：
--
作者：
Potluri,Tanvi;Fink,AshleyL;Sylvia,KristynE;Dhakal,Santosh;Vermillion,MeghanS;VomSteeg,Landon;Deshpande,Sharvari;Narasimhan,Harish;Klein,SabraL
疫苗诱导的免疫力随着年龄的增长而下降，这在男性和女性之间可能有所不同。利用接种单价A/Cal/09 H1N1疫苗前和接种后21天采集的人血清，我们评估了成人（18-45岁）和老年人（65岁以上）的细胞因子和抗体反应。接种疫苗后，成年女性比成年男性或老年女性产生更大的IL-6和抗体反应，女性抗体反应与雌二醇浓度呈正相关。为了测试雌性对流感病毒攻击的保护是否比雄性更强，我们用灭活的A/Cal/09 H1N1疫苗或不接种疫苗对成年（8-10周）和老年（68-70周）的雄性和雌性小鼠进行了启动和增强，并用漂移变体A/Cal/09病毒攻击。与未接种疫苗的小鼠相比，无论性别，接种疫苗的成年小鼠在攻击后发病率更低，肺病毒清除率更高。与接种疫苗的成年雄性小鼠相比，接种疫苗的成年雌性小鼠产生的抗体反应数量和质量都更高，而且更具保护性。在小鼠中，疫苗效力的性别差异随着年龄的增长而减弱。为了确定性类固醇在疫苗诱导的免疫反应中的作用，在接种疫苗前对成年小鼠进行了性腺切除术，并在动物亚群中替换激素（雌性雌二醇和雄性睾酮）。雌二醇增加了女性疫苗诱导的抗体反应，而睾酮降低了男性疫苗诱导的抗体反应。在小鼠和人类中，雌二醇升高对女性抗体反应和抗流感保护的益处随着年龄的增长而减弱。
Vaccine-induced immunity declines with age, which may differ between males and females. Using human sera collected before and 21 days after receipt of the monovalent A/Cal/09 H1N1 vaccine, we evaluated cytokine and antibody responses in adult (18–45 years) and aged (65+ years) individuals. After vaccination, adult females developed greater IL-6 and antibody responses than either adult males or aged females, with female antibody responses being positively associated with concentrations of estradiol. To test whether protection against influenza virus challenge was greater in females than males, we primed and boosted adult (8–10 weeks) and aged (68–70 weeks) male and female mice with an inactivated A/Cal/09 H1N1 vaccine or no vaccine and challenged with a drift variant A/Cal/09 virus. As compared with unvaccinated mice, vaccinated adult, but not aged, mice experienced less morbidity and better pulmonary viral clearance following challenge, regardless of sex. Vaccinated adult female mice developed antibody responses that were of greater quantity and quality and more protective than vaccinated adult males. Sex differences in vaccine efficacy diminished with age in mice. To determine the role of sex steroids in vaccine-induced immune responses, adult mice were gonadectomized and hormones (estradiol in females and testosterone in males) were replaced in subsets of animals before vaccination. Vaccine-induced antibody responses were increased in females by estradiol and decreased in males by testosterone. The benefit of elevated estradiol on antibody responses and protection against influenza in females is diminished with age in both mice and humans.