Synthetic neoglycopolymer-recombinant human collagen hybrids as biomimetic crosslinking agents in corneal tissue engineering
Synthetic neoglycopolymer-recombinant human collagen hybrids as biomimetic crosslinking agents in corneal tissue engineering
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DOI：
10.1016/j.biomaterials.2009.06.016
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Fogg, Deryn E.
中科院分区：
文献类型：
--
作者：
Merrett, Kimberley;Liu, Wenguang;Fogg, Deryn E.
饱和neoglycopolymers，通过串联开环易位聚合（ROMP =开环易位聚合）的碳水化合物官能化的降冰片烯的氢化制备，研究作为新的胶原交联剂在角膜组织工程。将neoglycopolymers作为胶原交联剂和糖胺聚糖（GAG）模拟物掺入重组人III型胶原（RHC III）中。这些复合材料的纯合成性质旨在降低对免疫和过敏反应的敏感性，并避免动物传染病的传播。胶原蛋白-neoglycopolymer生物材料对胶原酶诱导的生物降解的稳定性高于对照材料，即使用EDC/NHS（EDC = 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺; NHS = N-羟基琥珀酰亚胺）交联的RHC III复合材料。即使在这个概念验证阶段，neoglycopolymer水凝胶的热稳定性、耐酶性和渗透性也与这些完全优化的对照材料相当或上级这些对照材料，这些对照材料已经成功地进行了临床测试。抗拉强度足以移植，但低于优化的对照材料。(C)2009爱思唯尔有限公司保留所有权利。
Saturated neoglycopolymers, prepared via tandem ROMP-hydrogenation (ROMP = ring-opening metathesis polymerization) of carbohydrate-functionalized norbornenes, are investigated as novel collagen crosslinking agents in corneal tissue engineering. The neoglycopolymers were incorporated into recombinant human collagen type III (RHC III) as collagen crosslinking agents and glycosaminoglycan (GAG) mimics. The purely synthetic nature of these composites is designed to reduce susceptibility to immunological and allergic reactions, and to circumvent the transmission of animal infectious diseases. The collagen-neoglycopolymer biomaterials exhibit higher stability to collagenase-induced biodegradation than the control materials, composites of RHC III crosslinked using EDC/NHS (EDC = 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide; NHS = N-hydroxysuccinimide). Even at this proof of concept stage, the thermal stability, enzymatic resistance, and permeability of the neoglycopolymer hydrogels are comparable or superior to those of these fully optimized control materials, which have successfully been tested clinically. Tensile strength is adequate for transplantation, but lower than that of the optimized control materials. (C) 2009 Elsevier Ltd. All rights reserved.