Smooth muscle cell hypertrophy versus hyperplasia in hypertension.
Smooth muscle cell hypertrophy versus hyperplasia in hypertension.
复制标题
高血压中的平滑肌细胞肥大与增生。
DOI：
10.1073/pnas.78.12.7759
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Schwartz,SM
中科院分区：
文献类型：
--
作者：
Owens,GK;Rabinovitch,PS;Schwartz,SM
高血压动物的动脉比血压正常的对照动物的动脉平滑肌质量大。我们研究了平滑肌细胞肥大和增生对这种质量增加的贡献。通过（i）酶分散细胞的图像分析，（ii）组织切片的形态学评价，和（iii）分离细胞的蛋白质/细胞和肌动蛋白/细胞比率的生化测量获得的细胞大小测量结果表明，自发性高血压大鼠的平均细胞大小大于血压正常的Wistar-Kyoto和Sprague-Dawley对照组。自发性高血压大鼠的平均DNA/细胞比率也增加，而蛋白质/DNA比率没有差异。用流式显微荧光法和Feulgen-DNA显微光密度测定法对单个细胞核DNA含量进行分析，结果表明，超过20%的自发性高血压大鼠细胞为多倍体，而对照组细胞为多倍体的细胞不到10%。根据倍性和DNA含量，估计每厘米主动脉长度的细胞数，显示对照组和高血压大鼠之间没有差异。因此，平滑肌肥大单独解释了自发性高血压大鼠动脉平滑肌质量的增加。此外，这种细胞肥大伴随着核倍性的变化。高血压的这种核反应可能代表了与慢性高血压状态建立相关的固定变化。
Arteries of hypertensive animals have a greater mass of smooth muscle than those of normotensive controls. We examined the contribution of smooth muscle cell hypertrophy and hyperplasia to this increase in mass. Cell size measurements obtained by (i) image analysis of enzyme-dispersed cells, (ii) morphometric evaluation of tissue sections, and (iii) biochemical measures of protein/cell and actin/cell ratios on isolated cells showed that average cell size was greater in spontaneously hypertensive rats than in normotensive Wistar-Kyoto and Sprague-Dawley controls. Average DNA/cell ratios were also increased in spontaneously hypertensive rats while protein/DNA ratios were not different. Analysis of nuclear DNA content of individual cells by flow microfluorimetry and Feulgen-DNA microdensitometry measurements showed that greater than 20% of spontaneously hypertensive rats cells were polyploid while less than 10% of control cells were polyploid. Estimates of cell number per centimeter of aortic length, based on ploidy and DNA content, show no difference between control and hypertensive rats. Thus, smooth muscle hypertrophy alone accounts for the increased mass of smooth muscle in aortas of spontaneously hypertensive rats. Furthermore, this cellular hypertrophy is accompanied by a change in nuclear ploidy. This nuclear response in hypertension may represent a fixed change related to the establishment of a chronic hypertensive state.