rco-3, a gene involved in glucose transport and conidiation in Neurospora crassa.
rco-3, a gene involved in glucose transport and conidiation in Neurospora crassa.
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rco-3，粗糙脉孢菌中参与葡萄糖转运和分生孢子形成的基因。
DOI：
10.1093/genetics/146.2.499
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Ebbole,DJ
中科院分区：
文献类型：
--
作者：
Madi,L;McBride,SA;Bailey,LA;Ebbole,DJ
粗神经孢子虫的大孢子作用受许多环境因素的影响，包括生长生物体的营养状况。当真菌暴露在空气中时，通常观察到分生孢子的形成。然而，碳限制可以诱导浸没在曝气液体培养基中的菌丝的分生作用。之前已经分离出一种突变体，可以在不施加营养限制的情况下在水下培养中分生，并且现在已经克隆出了负责这种表型的基因（rco-3）。RCO3与糖转运基因超家族成员具有序列相似性，与酵母的葡萄糖转运蛋白相似性最大。与这种结构相似性一致，我们发现葡萄糖转运活性在突变体中发生了改变。然而，在含有替代碳源的培养基中，突变体的生长并不抑制潜水培养中的分生作用。该突变体的特性表明，RCO3是葡萄糖转运活性表达、葡萄糖调控基因表达和发育的一般碳抑制所必需的。
Macroconidiation inNeurospora crassais influenced by a number of environmental cues, including the nutritional status of the growing organism. Conidia formation is normally observed when the fungus is exposed to air. However, carbon limitation can induce conidiation in mycelia submerged in an aerated liquid medium. A mutant was previously isolated that could conidiate in submerged culture without imposing nutrient limitation and the gene responsible for this phenotype (rco-3) has now been cloned. RCO3 exhibits sequence similarity to members of the sugar transporter gene superfamily, with greatest similarity to glucose transporters of yeast. Consistent with this structural similarity, we find that glucose transport activity is altered in the mutant. However, growth of the mutant in media containing alternate carbon sources does not suppress conidiation in submerged culture. The properties of the mutant suggest that RCO3 is required for expression of glucose transport activity, glucose regulation of gene expression, and general carbon repression of development.