Effects of prostaglandin E1, ethanol and TSH on the adenylate cyclase activity of beef thyroid plasma membranes and cyclic AMP content of dog thyroid slices.
Effects of prostaglandin E1, ethanol and TSH on the adenylate cyclase activity of beef thyroid plasma membranes and cyclic AMP content of dog thyroid slices.
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前列腺素E1、乙醇和TSH对牛甲状腺质膜腺苷酸环化酶活性和狗甲状腺切片环AMP含量的影响。
DOI：
10.1210/endo-94-2-370
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发表时间：
1974
期刊：
影响因子：
4.8
通讯作者：
J. Field
中科院分区：
文献类型：
--
作者：
K. Mashiter;G. Mashiter;J. Field
前列腺素E_1(PGE_1)以剂量依赖的方式刺激牛肉甲状腺匀浆和分离质膜上腺苷环化酶的活性。1.4×10−7M PGE作用显著，1.4×10−5M PGE作用最大。较大剂量(1.4×10−，3M)的前列腺素E_1对细胞匀浆无影响，但可进一步刺激细胞膜腺苷环化酶。然而，这是由于用于溶解PGE1的乙醇。乙醇的刺激作用在0.25%(v/v)~5%浓度范围内呈线性关系。5%乙醇可刺激大鼠肝、肾质膜腺苷环化酶的活性，但不能刺激这些组织的匀浆或牛肉甲状腺的腺苷环化酶活性。乙醇(1%)对犬甲状腺切片中环3‘，5’-腺苷(CAMP)水平无影响。提示乙醇可通过膜扰动激活腺苷酸环化酶。阿司匹林(100μg/ml)和消炎痛(10μg/ml)均不能阻断促甲状腺激素对犬胸腺组织环磷酸腺苷和L−14C-葡萄糖氧化的影响.
Prostaglandin E1 (PGE1) stimulated adenylate cyclase activity in beef thyroid homogenates and isolated plasma membranes in a dose dependent manner. Effects were significant with, 1.4 × 10−7M PGE, and maximal with 1.4 × 10−5M PGE1. Larger doses (1.4 × 10−3M PGE1)had no effect on homogenates but caused a further stimulation of membrane adenylate cyclase. However, this was due to the ethanol used to dissolve the PGE1. The ethanol induced stimulation was linear over the concentration range 0.25% (v/v) t o 5%. Five percent ethanol stimulated adenylate cyclase activity in rat liver and kidney plasma membranes but not in homogenates of these tissues or of beef thyroid. Ethanol (1%) did not affect cyclic 3′,5′-adenosine monophosphate (cyclic AMP) levels in dog thyroid slices. It is suggested that ethanol can activate adenylate cyclase as a result of membrane perturbation. Neither aspirin (100 μg/ml), or indomethacin (10 μg/ml), blocked the effect of TSH on cyclic AMP levels or l−14C-glucose oxidation in dog thyro...